Search Results
How Much Physical Guidance is Needed to Orient Growing Axons in 3D Hydrogels?
2020, Rose, Jonas C., Gehlen, David B., Omidinia-Anarkoli, Abdolrahman, Fölster, Maaike, Haraszti, Tamás, Jaekel, Esther E., De Laporte, Laura
Directing cells is essential to organize multi-cellular organisms that are built up from subunits executing specific tasks. This guidance requires a precisely controlled symphony of biochemical, mechanical, and structural signals. While many guiding mechanisms focus on 2D structural patterns or 3D biochemical gradients, injectable material platforms that elucidate how cellular processes are triggered by defined 3D physical guiding cues are still lacking but crucial for the repair of soft tissues. Herein, a recently developed anisotropic injectable hybrid hydrogel (Anisogel) contains rod-shaped microgels that orient in situ by a magnetic field and has propelled studying 3D cell guidance. Here, the Anisogel is used to investigate the dependence of axonal guidance on microgel dimensions, aspect ratio, and distance. While large microgels result in high material anisotropy, they significantly reduce neurite outgrowth and thus the guidance efficiency. Narrow and long microgels enable strong axonal guidance with maximal outgrowth including cell sensing over distances of tens of micrometers in 3D. Moreover, nerve cells decide to orient inside the Anisogel within the first three days, followed by strengthening of the alignment, which goes along with oriented fibronectin deposition. These findings demonstrate the potential of the Anisogel to tune structural and mechanical parameters for specific applications. © 2020 The Authors. Published by Wiley-VCH GmbH
Tailoring the Cavity of Hollow Polyelectrolyte Microgels
2020, Wypysek, Sarah K., Scotti, Andrea, Alziyadi, Mohammed O., Potemkin, Igor I., Denton, Alan R., Richtering, Walter
The authors demonstrate how the size and structure of the cavity of hollow charged microgels may be controlled by varying pH and ionic strength. Hollow charged microgels based on N-isopropylacrylamide with ionizable co-monomers (itaconic acid) combine advanced structure with enhanced responsiveness to external stimuli. Structural advantages accrue from the increased surface area provided by the extra internal surface. Extreme sensitivity to pH and ionic strength due to ionizable moieties in the polymer network differentiates these soft colloidal particles from their uncharged counterparts, which sustain a hollow structure only at cross-link densities sufficiently high that stimuli sensitivity is reduced. Using small-angle neutron and light scattering, increased swelling of the network in the charged state accompanied by an expanded internal cavity is observed. Upon addition of salt, the external fuzziness of the microgel surface diminishes while the internal fuzziness grows. These structural changes are interpreted via Poisson–Boltzmann theory in the cell model. © 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Tuning the Volume Phase Transition Temperature of Microgels by Light
2021, Jelken, Joachim, Jung, Se-Hyeong, Lomadze, Nino, Gordievskaya, Yulia D., Kramarenko, Elena Yu., Pich, Andrij, Santer, Svetlana
Temperature-responsive microgels find widespread applications as soft materials for designing actuators in microfluidic systems, as carriers for drug delivery or catalysts, as functional coatings, and as adaptable sensors. The key property is their volume phase transition temperature, which allows for thermally induced reversible swelling/deswelling. It is determined by the gel's chemical structure as well as network topology and cannot be varied easily within one system. Here a paradigm change of this notion by facilitating a light-triggered reversible switching of the microgel volume in the range between 32 and 82 °C is suggested. Photo-sensitivity is introduced by photosensitive azobenzene containing surfactant, which forms a complex with microgels consisting of poly(N-isopropylacrylamide-co-acrylic acid) (PNIPAM-AAc) chains when assuming a hydrophobic trans-state, and prefers to leave the gel matrix in its cis-state. Using a similar strategy, it is demonstrated that at a fixed temperature, for example, 37 °C, one can reversibly change the microgel radius by a factor of 3 (7–21 µm) by irradiating either with UV (collapsed state) or green light (swollen state). It is envisaged that the possibility to deploy a swift external means of adapting the swelling behavior of microgels may impact and redefine the latter's application across all fields. © 2021 The Authors. Advanced Functional Materials published by Wiley-VCH GmbH
Zwitterionic Nanogels and Microgels: An Overview on Their Synthesis and Applications
2021, Saha, Pabitra, Ganguly, Ritabrata, Li, Xin, Das, Rohan, Singha, Nikhil K., Pich, Andrij
Zwitterionic polymers by virtue of their unique chemical and physical attributes have attracted researchers in recent years. The simultaneous presence of positive and negative charges in the same repeat unit renders them of various interesting properties such as superhydrophilicity, which has significantly broadened their scope for being used in different applications. Among polyzwitterions of different architectures, micro- and/or nano-gels have started receiving attention only until recently. These 3D cross-linked colloidal structures show peculiar characteristics in context to their solution properties, which are attributable either to the comonomers present or the presence of different electrolytes and biological specimens. In this review, a concise yet detailed account is provided of the different synthetic techniques and application domains of zwitterion-based micro- and/or nanogels that have been explored in recent years. Here, the focus is kept solely on the “polybetaines,” which have garnered maximum research interest and remain the extensively studied polyzwitterions in literature. While their vast application potential in the biomedical sector is being detailed here, some other areas of scope such as using them as microreactors for the synthesis of metal nanoparticles or making smart membranes for water-treatment are discussed in this minireview as well.