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- ItemOn the Resistances of a Slurry Electrode Vanadium Redox Flow Battery(Weinheim : Wiley-VCH, 2020) Percin, Korcan; van der Zee, Bart; Wessling, MatthiasWe studied the half-cell performance of a slurry-based vanadium redox flow battery via the polarization and electrochemical impedance spectroscopy methods. First, the conductive static mixers are examined and lower ohmic and diffusion resistances are shown. Further analyses of the slurry electrodes for the catholyte (VO2+−VO2 +) and anolyte (V3+−V2+) are presented for the graphite powder slurry containing up to 15.0 wt.% particle content. Overall, the anolyte persists as the more resistive half-cell, while ohmic and diffusion-related limitations are the dominating resistances for both electrolytes. The battery is further improved by the addition of Ketjen black nanoparticles, which results in lower cell resistances. The best results are achieved when 0.5 wt.% Ketjen black nanoparticles are dispersed with graphite powder since the addition of nanoparticles reduces ohmic, charge transfer and mass diffusion resistances by improving particle-particle dynamics. The results prove the importance of understanding resistances in a slurry electrode system. © 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.
- ItemSortase-Mediated Ligation of Purely Artificial Building Blocks(Basel : MDPI, 2018) Dai, Xiaolin; Mate, Diana M.; Glebe, Ulrich; Mirzaei Garakani, Tayebeh; Körner, Andrea; Schwaneberg, Ulrich; Böker, AlexanderSortase A (SrtA) from Staphylococcus aureus has been often used for ligating a protein with other natural or synthetic compounds in recent years. Here we show that SrtA-mediated ligation (SML) is universally applicable for the linkage of two purely artificial building blocks. Silica nanoparticles (NPs), poly(ethylene glycol) and poly(N-isopropyl acrylamide) are chosen as synthetic building blocks. As a proof of concept, NP–polymer, NP–NP, and polymer–polymer structures are formed by SrtA catalysis. Therefore, the building blocks are equipped with the recognition sequence needed for SrtA reaction—the conserved peptide LPETG—and a pentaglycine motif. The successful formation of the reaction products is shown by means of transmission electron microscopy (TEM), matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS), and dynamic light scattering (DLS). The sortase catalyzed linkage of artificial building blocks sets the stage for the development of a new approach to link synthetic structures in cases where their synthesis by established chemical methods is complicated.
- Item3D-Printing of Structure-Controlled Antigen Nanoparticles for Vaccine Delivery(Columbus, Ohio : American Chemical Soc., 2020) Nishiguchi, Akihiro; Shima, Fumiaki; Singh, Smriti; Akashi, Mitsuru; Moeller, MartinTargeted delivery of antigens to immune cells using micro/nanocarriers may serve as a therapeutic application for vaccination. However, synthetic carriers have potential drawbacks including cytotoxicity, low encapsulation efficiency of antigen, and lack of a morphological design, which limit the translation of the delivery system to clinical use. Here, we report a carrier-free and three-dimensional (3D)-shape-designed antigen nanoparticle by multiphoton lithography-based 3D-printing. This simple, versatile 3D-printing approach provides freedom for the precise design of particle shapes with a nanoscale resolution. Importantly, shape-designed antigen nanoparticles with distinct aspect ratios show shape-dependent immune responses. The 3D-printing approach for the rational design of nanomaterials with increasing safety, complexity, and efficacy offers an emerging platform to develop vaccine delivery systems and mechanistic understanding.
- ItemBiofunctionalized zinc peroxide (ZnO2) nanoparticles as active oxygen sources and antibacterial agents(London : RSC Publishing, 2017) Bergs, Christian; Brück, Lisa; Rosencrantz, Ruben R.; Conrads, Georg; Elling, Lothar; Pich, AndrijOxygen is one of the most important substances for physiological reactions and metabolisms in biological systems. Through the tailored design of oxygen-releasing materials it might be possible to control different biological processes. In this work we synthesized for the first time zinc peroxide nanoparticles with controlled sizes and biofunctionalized surfaces using a one-step reaction procedure. The zinc peroxide nanoparticles were obtained with tunable sizes (between 4.0 ± 1.2 nm and 9.4 ± 5.2 nm) and were decorated with glucose 1-phosphate (Glc-1P). The specific interaction of the phosphate function of Glc-1P with the nanoparticle surface was monitored by solid state 31P-NMR and zeta-potential measurements. Furthermore, using fluorescence measurements we demonstrated that anchored glucose molecules on the nanoparticle surface are accessible for specific interactions with lectins. It could be shown that these interactions strongly depend on the amount of Glc-1P attached to the nanoparticle surface. Additionally it was demonstrated that the oxygen release from biofunctionalized zinc peroxide nanoparticles could be tuned according to the chemical composition of the nanoparticles and the pH of the aqueous solution. The antibacterial efficiency of the synthesized nanoparticles against Enterococcus faecalis, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Prevotella intermedia was evaluated by determination of minimal bactericidal concentration (MIC).
- ItemSelective hydrogenation of fluorinated arenes using rhodium nanoparticles on molecularly modified silica(London : RSC Publ., 2020) Kacem, Souha; Emondts, Meike; Bordet, Alexis; Leitner, WalterThe production of fluorinated cyclohexane derivatives is accomplished through the selective hydrogenation of readily available fluorinated arenes using Rh nanoparticles on molecularly modified silica supports (Rh@Si-R) as highly effective and recyclable catalysts. The catalyst preparation comprises grafting non-polar molecular entities on the SiO2 surface generating a hydrophobic environment for controlled deposition of well-defined rhodium particles from a simple organometallic precursor. A broad range of fluorinated cyclohexane derivatives was shown to be accessible with excellent efficacy (0.05-0.5 mol% Rh, 10-55 bar H2, 80-100 °C, 1-2 h), including industrially relevant building blocks. Addition of CaO as scavenger for trace amounts of HF greatly improves the recyclability of the catalytic system and prevents the risks associated to the presence of HF, without compromising the activity and selectivity of the reaction. © The Royal Society of Chemistry.