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- ItemThe First Report of mcr-1-Carrying Escherichia coli Originating from Animals in Serbia(Basel : MDPI, 2021) Mišić, Dušan; Kiskaroly, Ferenc; Szostak, Michael P.; Cabal, Adriana; Ruppitsch, Werner; Bernreiter-Hofer, Tanja; Milovanovic, Viktoria; Feßler, Andrea T.; Allerberger, Franz; Spergser, Joachim; Müller, Elke; Schwarz, Stefan; Braun, Sascha D.; Monecke, Stefan; Ehricht, Ralf; Korus, Maciej; Benković, Damir; Korzeniowska, Malgorzata; Loncaric, IgorThe aim of this study was continuous monitoring of the presence of mcr-1 to mcr-5 genes in Enterobacterales isolated from cattle, pigs, and domestic poultry at intensive breeding facilities in Northern Vojvodina, Serbia, from 1 January 1 to 1 October 2020. Out of 2167 examined samples, mcr-1 was observed in five E. coli isolates originating from healthy turkeys. Four isolates belonged to the phylogenetic group B1, and one isolate to the phylogenetic group A. Detected E. coli serogenotypes (somatic O and flagellar H antigens) were O8:H25 and O29:H25. Core-genome multi-locus sequence typing (cgMLST) revealed three ST58 isolates clustering together in Clonal Complex (CC) 155 and two singletons of ST641-CC86 and ST410-CC23, respectively. Clonotyping revealed CH4-32 (n = 3), CH6-53 (n = 1) and CH4-24 (n = 1). In all isolates, the mcr-1 gene was located on a large IncX4 replicon type plasmid. Eight virulence-associated genes (VAGs) typical of avian pathogenic E. coli (APEC) (fyuA, fimH, hlyF, iss, ompT, sitA, traT, iroN) were detected in four isolates. These isolates were investigated for susceptibility to four biocides and revealed MIC values of 0.125% for glutardialdehyde, of 0.00003-0.00006% for chlorohexidine, of 4-6% for isopropanol and of 0.001-0.002% for benzalkonium chloride. All obtained MIC values of the tested biocides were comparable to the reference strain, with no indication of possible resistance. This is the first report of mcr-1.1-carrying E. coli from Serbia. Although only samples from turkeys were mcr-positive in this study, continuous monitoring of livestock samples is advised to prevent a spill-over from animals to humans.
- ItemResponses of fish and invertebrates to floods and droughts in Europe(Hoboken, NJ : Wiley, 2016) Piniewsk, M.; Prudhomme, C.; Acreman, M.C.; Tylec, L.; Oglęcki, P.; Okruszko, T.Floods and droughts, two opposite natural components of streamflow regimes, are known to regulate population size and species diversity. Quantifiable measures of these disturbances and their subsequent ecological responses are needed to synthesize the knowledge on flow–ecosystem relationships. This study for the first time combines the systematic review approach used to collect evidence on the ecological responses to floods and droughts in Europe with the statistical methods used to quantify the extreme events severity. Out of 854 publications identified in literature search, 54 papers were retained after screening and eligibility checks, providing in total 82 case studies with unique extreme event—ecological response associations for which data were extracted. In this way, a database with metadata of case studies that can be explored with respect to various factors was constructed. This study pinpointed the research gaps where little evidence could be synthesized, for example, drought event studies and fish studies. It was demonstrated that in many cases the studied metrics (abundance, density, richness, and diversity) showed statistically significant decreases after or during the event occurrence. The responses in invertebrate density and richness were in general more negative than the corresponding responses in fish. Biota resistance to floods was found to be lower than the resistance to droughts. The severity of extreme events was not found to be an important factor influencing ecological metrics, although this analysis was often hampered by insufficient number of case studies. Conceivably, other factors could mask any existing relationships between disturbance severity and biotic response.