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- ItemDeep learning as phase retrieval tool for CARS spectra(Washington, DC : Soc., 2020) Houhou, Rola; Barman, Parijat; Schmitt, Micheal; Meyer, Tobias; Popp, Juergen; Bocklitz, ThomasFinding efficient and reliable methods for the extraction of the phase in optical measurements is challenging and has been widely investigated. Although sophisticated optical settings, e.g. holography, measure directly the phase, the use of algorithmic methods has gained attention due to its efficiency, fast calculation and easy setup requirements. We investigated three phase retrieval methods: the maximum entropy technique (MEM), the Kramers-Kronig relation (KK), and for the first time deep learning using the Long Short-Term Memory network (LSTM). LSTM shows superior results for the phase retrieval problem of coherent anti-Stokes Raman spectra in comparison to MEM and KK. © 2020 OSA - The Optical Society. All rights reserved.
- ItemStatistical Analysis of Scanning Fluorescence Correlation Spectroscopy Data Differentiates Free from Hindered Diffusion(Washington, DC : Soc., 2018-7-20) Schneider, Falk; Waithe, Dominic; Lagerholm, B. Christoffer; Shrestha, Dilip; Sezgin, Erdinc; Eggeling, Christian; Fritzsche, MarcoCells rely on versatile diffusion dynamics in their plasma membrane. Quantification of this often heterogeneous diffusion is essential to the understanding of cell regulation and function. Yet such measurements remain a major challenge in cell biology, usually due to low sampling throughput, a necessity for dedicated equipment, sophisticated fluorescent label strategies, and limited sensitivity. Here, we introduce a robust, broadly applicable statistical analysis pipeline for large scanning fluorescence correlation spectroscopy data sets, which uncovers the nanoscale heterogeneity of the plasma membrane in living cells by differentiating free from hindered diffusion modes of fluorescent lipid and protein analogues.
- ItemSide-view holographic endomicroscopy via a custom-terminated multimode fibre(Washington, DC : Soc., 2021) Silveira, Beatriz M.; Pikálek, Tomáš; Stibůrek, Miroslav; Ondráčková, Petra; Jákl, Petr; Leite, Ivo T.; Čižmár, TomášMicroendoscopes based on optical fibres have recently come to the fore as promising candidates allowing in-vivo observations of otherwise inaccessible biological structures in animal models. Despite being still in its infancy, imaging can now be performed at the tip of a single multimode fibre, by relying on powerful holographic methods for light control. Fibre based endoscopy is commonly performed en face, resulting in possible damage of the specimen owing to the direct contact between the distal end of the probe and target. On this ground, we designed an all-fibre probe with an engineered termination that reduces compression and damage to the tissue under investigation upon probe insertion. The geometry of the termination brings the field of view to a plane parallel to the fibre’s longitudinal direction, conveying the probe with off-axis imaging capabilities. We show that its focusing ability also benefits from a higher numerical aperture, resulting in imaging with increased spatial resolution. The effect of probe insertion was investigated inside a tissue phantom comprising fluorescent particles suspended in agarose gel, and a comparison was established between the novel side-view probe and the standard en face fibre probe. This new concept paves the way to significantly less invasive deep-tissue imaging.
- ItemLaser-induced spatially-selective tailoring of high-index dielectric metasurfaces(Washington, DC : Soc., 2020) Berzinš, Jona; Indrišiūna, Simonas; Fasold, Stefan; Steinert, Michael; Žukovskaja, Olga; Cialla-May, Dana; Gečys, Paulius; Bäumer, Stefan M.B.; Pertsch, Thomas; Setzpfandt, FrankOptically resonant high-index dielectric metasurfaces featuring Mie-type electric and magnetic resonances are usually fabricated by means of planar technologies, which limit the degrees of freedom in tunability and scalability of the fabricated systems. Therefore, we propose a complimentary post-processing technique based on ultrashort (= 10 ps) laser pulses. The process involves thermal effects: crystallization and reshaping, while the heat is localized by a high-precision positioning of the focused laser beam. Moreover, for the first time, the resonant behavior of dielectric metasurface elements is exploited to engineer a specific absorption profile, which leads to a spatially-selective heating and a customized modification. Such technique has the potential to reduce the complexity in the fabrication of non-uniform metasurface-based optical elements. Two distinct cases, a spatial pixelation of a large-scale metasurface and a height modification of metasurface elements, are explicitly demonstrated. © 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
- ItemSingle-shot interferometric measurement of pulse-to-pulse stability of absolute phase using a time-stretch technique(Washington, DC : Soc., 2021) Kudelin, Igor; Sugavanam, Srikanth; Chernysheva, MariaMeasurement of the absolute phase of ultrashort optical pulses in real-time is crucial for various applications, including frequency comb and high-field physics. Modern single-shot techniques, such as dispersive Fourier transform and time-lens, make it possible to investigate non-repetitive spectral dynamics of ultrashort pulses yet do not provide the information on absolute phase. In this work, we demonstrate a novel approach to characterise single-shot pulse-to-pulse stability of the absolute phase with the acquisition rate of 15 MHz. The acquisition rate, limited by the repetition rate of the used free-running mode-locked Erbium-doped fibre laser, substantially exceeds one of the traditional techniques. The method is based on the time-stretch technique. It exploits a simple all-fibre Mach-Zehnder interferometric setup with a remarkable resolution of ∼7.3 mrad. Using the proposed method, we observed phase oscillations in the output pulses governed by fluctuations in the pulse intensity due to Kerr-induced self-phase modulation at frequencies peaked at 4.6 kHz. As a proof-of-concept application of the demonstrated interferometric methodology, we evaluated phase behaviour during vibration exposure on the laser platform. The results propose a new view on the phase measurements that provide a novel avenue for numerous sensing applications with MHz data frequencies.
- ItemOrders of magnitude loss reduction in photonic bandgap fibers by engineering the core surround(Washington, DC : Soc., 2021) Upendar, S.; Ando, R.F.; Schmidt, M.A.; Weiss, T.We demonstrate how to reduce the loss in photonic bandgap fibers by orders of magnitude by varying the radius of the corner strands in the core surround. As a fundamental working principle we find that changing the corner strand radius can lead to backscattering of light into the fiber core. Selecting an optimal corner strand radius can thus reduce the loss of the fundamental core mode in a specific wavelength range by almost two orders of magnitude when compared to an unmodified cladding structure. Using the optimal corner radius for each transmission window, we observe the low-loss behavior for the first and second bandgaps, with the losses in the second bandgap being even lower than that of the first one. Our approach of reducing the confinement loss is conceptually applicable to all kinds of photonic bandgap fibers including hollow core and all-glass fibers as well as on-chip light cages. Therefore, our concept paves the way to low-loss light guidance in such systems with substantially reduced fabrication complexity.
- ItemFast, Label-Free Tracking of Single Viruses and Weakly Scattering Nanoparticles in a Nanofluidic Optical Fiber(Washington, DC : Soc., 2015) Faez, Sanli; Lahini, Yoav; Weidlich, Stefan; Garmann, Rees F.; Wondraczek, Katrin; Zeisberger, Matthias; Schmidt, Markus A.; Orrit, Michel; Manoharan, Vinothan N.High-speed tracking of single particles is a gateway to understanding physical, chemical, and biological processes at the nanoscale. It is also a major experimental challenge, particularly for small, nanometer-scale particles. Although methods such as confocal or fluorescence microscopy offer both high spatial resolution and high signal-to-background ratios, the fluorescence emission lifetime limits the measurement speed, while photobleaching and thermal diffusion limit the duration of measurements. Here we present a tracking method based on elastic light scattering that enables long-duration measurements of nanoparticle dynamics at rates of thousands of frames per second. We contain the particles within a single-mode silica fiber having a subwavelength, nanofluidic channel and illuminate them using the fiber's strongly confined optical mode. The diffusing particles in this cylindrical geometry are continuously illuminated inside the collection focal plane. We show that the method can track unlabeled dielectric particles as small as 20 nm as well as individual cowpea chlorotic mottle virus (CCMV) virions-26 nm in size and 4.6 megadaltons in mass-at rates of over 3 kHz for durations of tens of seconds. Our setup is easily incorporated into common optical microscopes and extends their detection range to nanometer-scale particles and macromolecules. The ease-of-use and performance of this technique support its potential for widespread applications in medical diagnostics and micro total analysis systems.
- ItemUltrafast intermodal third harmonic generation in a liquid core step-index fiber filled with C2Cl4(Washington, DC : Soc., 2020) Schaarschmidt, Kay; Kobelke, Jens; Nolte, Stefan; Meyer, Tobias; Schmidt, Markus A.Third harmonic generation in a circular liquid core step-index fiber filled with a highly transparent inorganic solvent is demonstrated experimentally using ultrafast pump pulses of different durations in the telecom domain for the first time. Specifically we achieve intermodal phase matching to the HE13 higher order mode at the harmonic wavelength and found clear indications of a non-instantaneous molecular contribution to the total nonlinearity in the spectral broadening of the pump. Spectral power evolution and efficiency of the conversion process is studied for all pulse parameters, while we found the greatest photon yield for the longest pulses as well as an unexpected blue-shift of the third harmonic wavelength with increasing pump power. Our results provide the basis for future studies aiming at using this tunable fiber platform with a sophisticated nonlinear response in the context of harmonic generation. © 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
- ItemNegative curvature hollow core fiber sensor for the measurement of strain and temperature(Washington, DC : Soc., 2021) Ferreira, Marta S.; Bierlich, Jörg; Kobelke, Jens; Pinto, João L.; Wondraczek, KatrinThree different types of strain and temperature sensors based on negative curvature hollow core fiber (NCHCF) are proposed. Each sensor is produced by splicing a small section of the NCHCF between two sections of single mode fiber. Different types of interferometers are obtained simply by changing the splicing conditions. The first sensor consists on a single Fabry-Perot interferometer (FPI). The remaining two configurations are attained with the same sensing structure, depending on its position in relation to the interrogation setup. Thus, a double FPI or a hybrid sensor, the latter being composed by an FPI and a Michelson interferometer, are formed. The inline sensors are of submillimeter size, thus enabling nearly punctual measurements.
- ItemTime-averaged image projection through a multimode fiber(Washington, DC : Soc., 2021) Boonzajer Flaes, Dirk; Štolzová, Hana; Čižmár, TomášMany disciplines, ranging from lithography to opto-genetics, require high-fidelity image projection. However, not all optical systems can display all types of images with equal ease. Therefore, the image projection quality is dependent on the type of image. In some circumstances, this can lead to a catastrophic loss of intensity or image quality. For complex optical systems, it may not be known in advance which types of images pose a problem. Here we show a new method called Time-Averaged image Projection (TAP), allowing us to mitigate these limitations by taking the entire image projection system into account despite its complexity and building the desired intensity distribution up from multiple illumination patterns. Using a complex optical setup, consisting of a wavefront shaper and a multimode optical fiber illuminated by coherent light, we succeeded to suppress any speckle-related background. Further, we can display independent images at multiple distances simultaneously, and alter the effective sharpness depth through the algorithm. Our results demonstrate that TAP can significantly enhance the image projection quality in multiple ways. We anticipate that our results will greatly complement any application in which the response to light irradiation is relatively slow (one microsecond with current technology) and where high-fidelity spatial distribution of optical power is required.