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Type: article × Subject: Anaerobic digestion ×

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    Metaproteome analysis reveals that syntrophy, competition, and phage-host interaction shape microbial communities in biogas plants
    (London : Biomed Central, 2019) Heyer, R.; Schallert, K.; Siewert, C.; Kohrs, F.; Greve, J.; Maus, I.; Klang, J.; Klocke, M.; Heiermann, M.; Hoffmann, M.; Püttker, S.; Calusinska, M.; Zoun, R.; Saake, G.; Benndorf, D.; Reichl, U.
    Background: In biogas plants, complex microbial communities produce methane and carbon dioxide by anaerobic digestion of biomass. For the characterization of the microbial functional networks, samples of 11 reactors were analyzed using a high-resolution metaproteomics pipeline. Results: Examined methanogenesis archaeal communities were either mixotrophic or strictly hydrogenotrophic in syntrophy with bacterial acetate oxidizers. Mapping of identified metaproteins with process steps described by the Anaerobic Digestion Model 1 confirmed its main assumptions and also proposed some extensions such as syntrophic acetate oxidation or fermentation of alcohols. Results indicate that the microbial communities were shaped by syntrophy as well as competition and phage-host interactions causing cell lysis. For the families Bacillaceae, Enterobacteriaceae, and Clostridiaceae, the number of phages exceeded up to 20-fold the number of host cells. Conclusion: Phage-induced cell lysis might slow down the conversion of substrates to biogas, though, it could support the growth of auxotrophic microbes by cycling of nutrients. © 2019 The Author(s).
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    The role of petrimonas mucosa ING2-E5at in mesophilic biogas reactor systems as deduced from multiomics analyses
    (Basel : MDPI AG, 2020) Maus, Irena; Tubbesing, Tom; Wibberg, Daniel; Heyer, Robert; Hassa, Julia; Tomazetto, Geizecler; Huang, Liren; Bunk, Boyke; Spröer, Cathrin; Benndorf, Dirk; Zverlov, Vladimir; Pühler, Alfred; Klocke, Michael; Sczyrba, Alexander; Schlüter, Andreas
    Members of the genera Proteiniphilum and Petrimonas were speculated to represent indicators reflecting process instability within anaerobic digestion (AD) microbiomes. Therefore, Petrimonas mucosa ING2-E5AT was isolated from a biogas reactor sample and sequenced on the PacBio RSII and Illumina MiSeq sequencers. Phylogenetic classification positioned the strain ING2-E5AT in close proximity to Fermentimonas and Proteiniphilum species (family Dysgonomonadaceae). ING2-E5AT encodes a number of genes for glycosyl-hydrolyses (GH) which are organized in Polysaccharide Utilization Loci (PUL) comprising tandem susCD-like genes for a TonB-dependent outer-membrane transporter and a cell surface glycan-binding protein. Different GHs encoded in PUL are involved in pectin degradation, reflecting a pronounced specialization of the ING2-E5AT PUL systems regarding the decomposition of this polysaccharide. Genes encoding enzymes participating in amino acids fermentation were also identified. Fragment recruitments with the ING2-E5AT genome as a template and publicly available metagenomes of AD microbiomes revealed that Petrimonas species are present in 146 out of 257 datasets supporting their importance in AD microbiomes. Metatranscriptome analyses of AD microbiomes uncovered active sugar and amino acid fermentation pathways for Petrimonas species. Likewise, screening of metaproteome datasets demonstrated expression of the Petrimonas PUL-specific component SusC providing further evidence that PUL play a central role for the lifestyle of Petrimonas species. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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    Indicative Marker Microbiome Structures Deduced from the Taxonomic Inventory of 67 Full-Scale Anaerobic Digesters of 49 Agricultural Biogas Plants
    (Basel : MDPI, 2021) Hassa, Julia; Klang, Johanna; Benndorf, Dirk; Pohl, Marcel; Hülsemann, Benedikt; Mächtig, Torsten; Effenberger, Mathias; Pühler, Alfred; Schlüter, Andreas; Theuerl, Susanne
    There are almost 9500 biogas plants in Germany, which are predominantly operated with energy crops and residues from livestock husbandry over the last two decades. In the future, biogas plants must be enabled to use a much broader range of input materials in a flexible and demand-oriented manner. Hence, the microbial communities will be exposed to frequently varying process conditions, while an overall stable process must be ensured. To accompany this transition, there is the need to better understand how biogas microbiomes respond to management measures and how these responses affect the process efficiency. Therefore, 67 microbiomes originating from 49 agricultural, full-scale biogas plants were taxonomically investigated by 16S rRNA gene amplicon sequencing. These microbiomes were separated into three distinct clusters and one group of outliers, which are characterized by a specific distribution of 253 indicative taxa and their relative abundances. These indicative taxa seem to be adapted to specific process conditions which result from a different biogas plant operation. Based on these results, it seems to be possible to deduce/assess the general process condition of a biogas digester based solely on the microbiome structure, in particular on the distribution of specific indicative taxa, and without knowing the corresponding operational and chemical process parameters. Perspectively, this could allow the development of detection systems and advanced process models considering the microbial diversity.
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    Greenhouse gas emissions from broiler manure treatment options are lowest in well-managed biogas production
    (Amsterdam [u.a.] : Elsevier Science, 2020) Kreidenweis, Ulrich; Breier, Jannes; Herrmann, Christiane; Libra, Judy; Prochnow, Annette
    The production of broiler meat has increased significantly in the last decades in Germany and worldwide, and is projected to increase further in the future. As the number of animals raised increases, so too does the amount of manure produced. The identification of manure treatment options that cause low greenhouse gas emissions becomes ever more important. This study compares four treatment options for broiler manure followed by field spreading: storage before distribution, composting, anaerobic digestion in a biogas plant and production of biochar. For these options potential direct and indirect greenhouse gas emissions were assessed for the situation in Germany. Previous analyses have shown that greenhouse gas balances of manure management are often strongly influenced by a small number of processes. Therefore, in this study major processes were represented with several variants and the sensitivity of model results to different management decisions and uncertain parameters was assessed. In doing so, correlations between processes were considered, in which higher emissions earlier on in the process chain reduce emissions later. The results show that biogas production from broiler manure leads to the lowest greenhouse gas emissions in most of the analysed cases, mainly due to the emission savings related to the substitution of mineral fertilizers and the production of electricity. Pyrolysis of the manure and subsequent field spreading as a soil amendment can lead to similarly low emissions due to the long residence time of the biochar, and may even be the better option than poorly managed biogas production. Composting is the treatment option resulting in highest emissions of greenhouse gases, due to high ammonia volatilization, and is likely worse than untreated storage in this respect. These results are relatively insensitive to the length of transport required for field spreading, but high uncertainties are associated with the use of emission factors.