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    Engineering robust cellulases for tailored lignocellulosic degradation cocktails
    (Basel : MDPI AG, 2020) Contreras, Francisca; Pramanik, Subrata; Rozhkova, Aleksandra M.; Zorov, Ivan N.; Korotkova, Olga; Sinitsyn, Arkady P.; Schwaneberg, Ulrich; Davari, Mehdi D.
    Lignocellulosic biomass is a most promising feedstock in the production of second-generation biofuels. Efficient degradation of lignocellulosic biomass requires a synergistic action of several cellulases and hemicellulases. Cellulases depolymerize cellulose, the main polymer of the lignocellulosic biomass, to its building blocks. The production of cellulase cocktails has been widely explored, however, there are still some main challenges that enzymes need to overcome in order to develop a sustainable production of bioethanol. The main challenges include low activity, product inhibition, and the need to perform fine-tuning of a cellulase cocktail for each type of biomass. Protein engineering and directed evolution are powerful technologies to improve enzyme properties such as increased activity, decreased product inhibition, increased thermal stability, improved performance in non-conventional media, and pH stability, which will lead to a production of more efficient cocktails. In this review, we focus on recent advances in cellulase cocktail production, its current challenges, protein engineering as an efficient strategy to engineer cellulases, and our view on future prospects in the generation of tailored cellulases for biofuel production. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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    Global scenarios of irrigation water abstractions for bioenergy production: a systematic review
    (Munich : EGU, 2021) Stenzel, Fabian; Gerten, Dieter; Hanasaki, Naota
    Many scenarios of future climate evolution and its anthropogenic drivers include considerable amounts of bioenergy as a fuel source, as a negative emission technology, and for providing electricity. The associated freshwater abstractions for irrigation of dedicated biomass plantations might be substantial and therefore potentially increase water limitation and stress in affected regions; however, assumptions and quantities of water use provided in the literature vary strongly. This paper reviews existing global assessments of freshwater abstractions for bioenergy production and puts these estimates into the context of scenarios of other water-use sectors. We scanned the available literature and (out of 430 initial hits) found 16 publications (some of which include several bioenergy-water-use scenarios) with reported values on global irrigation water abstractions for biomass plantations, suggesting water withdrawals in the range of 128.4 to 9000 km3 yr−1, which would come on top of (or compete with) agricultural, industrial, and domestic water withdrawals. To provide an understanding of the origins of this large range, we present the diverse underlying assumptions, discuss major study differences, and calculate an inverse water-use efficiency (iwue), which facilitates comparison of the required freshwater amounts per produced biomass harvest. We conclude that due to the potentially high water demands and the tradeoffs that might go along with them, bioenergy should be an integral part of global assessments of freshwater demand and use. For interpreting and comparing reported estimates of possible future bioenergy water abstractions, full disclosure of parameters and assumptions is crucial. A minimum set should include the complete water balances of bioenergy production systems (including partitioning of blue and green water), bioenergy crop species and associated water-use efficiencies, rainfed and irrigated bioenergy plantation locations (including total area and meteorological conditions), and total biomass harvest amounts. In the future, a model intercomparison project with standardized parameters and scenarios would be helpful.
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    System complexity and policy integration challenges: The Brazilian Energy- Water-Food Nexus
    (Amsterdam [u.a.] : Elsevier Science, 2019) Mercure, J.-F.; Paim, M.A.; Bocquillon, P.; Lindner, S.; Salas, P.; Martinelli, P.; Berchin, I.I.; de Andrade Guerra, J.B.S.O; Derani, C.; de Albuquerque Junior, C.L.; Ribeiro, J.M.P.; Knobloch, F.; Pollitt, H.; Edwards, N.R.; Holden, P.B.; Foley, A.; Schaphoff, S.; Faraco, R.A.; Vinuales, J.E.
    The Energy-Water-Food Nexus is one of the most complex sustainability challenges faced by the world. This is particularly true in Brazil, where insufficiently understood interactions within the Nexus are contributing to large-scale deforestation and land-use change, water and energy scarcity, and increased vulnerability to climate change. The reason is a combination of global environmental change and global economic change, putting unprecedented pressures on the Brazilian environment and ecosystems. In this paper, we identify and discuss the main Nexus challenges faced by Brazil across sectors (e.g. energy, agriculture, water) and scales (e.g. federal, state, municipal). We use four case studies to explore all nodes of the Nexus. For each, we analyse data from economic and biophysical modelling sources in combination with an overview of the legislative and policy landscape, in order to identify governance shortcomings in the context of growing challenges. We analyse the complex interdependence of developments at the global and local (Brazilian) levels, highlighting the impact of global environmental and economic change on Brazil and, conversely, that of developments in Brazil for other countries and the world. We conclude that there is a need to adjust the scientific approach to these challenges as an enabling condition for stronger science-policy bridges for sustainability policy-making. © 2019 The Author(s)