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- ItemNeutrophil extracellular trap formation is elicited in response to cold physical plasma(Hoboken, NJ : Wiley, 2016) Bekeschus, Sander; Winterbourn, VChristine C.; Kolata, Julia; Masur, Kai; Hasse, Sybille; Bröker, Barbara M.; Parker, Heather A.Cold physical plasma is an ionized gas with a multitude of components, including hydrogen peroxide and other reactive oxygen and nitrogen species. Recent studies suggest that exposure of wounds to cold plasma may accelerate healing. Upon wounding, neutrophils are the first line of defense against invading microorganisms but have also been identified to play a role in delayed healing. In this study, we examined how plasma treatment affects the functions of peripheral blood neutrophils. Plasma treatment induced oxidative stress, as assessed by the oxidation of intracellular fluorescent redox probes; reduced metabolic activity; but did not induce early apoptosis. Neutrophil oxidative burst was only modestly affected after plasma treatment, and the killing of Pseudomonas aeruginosa and Staphylococcus aureus was not significantly affected. Intriguingly, we found that plasma induced profound extracellular trap formation. This was inhibited by the presence of catalase during plasma treatment but was not replicated by adding an equivalent concentration of hydrogen peroxide. Plasma-induced neutrophil extracellular trap formation was not dependent on the activity of myeloperoxidase or NADPH oxidase 2 but seemed to involve short-lived molecules. The amount of DNA release and the time course after plasma treatment were similar to that with the common neutrophil extracellular trap inducer PMA. After neutrophil extracellular traps had formed, concentrations of IL-8 were also significantly increased in supernatants of plasma-treated neutrophils. Both neutrophil extracellular traps and IL-8 release may aid antimicrobial activity and spur inflammation at the wound site. Whether this aids or exacerbates wound healing needs to be tested.
- ItemPoly (hexamethylene biguanide), adsorbed onto Ti-Al-V alloys, kills slime-producing Staphylococci and Pseudomonas aeruginosa without inhibiting SaOs-2 cell differentiation(Hoboken, NJ : Wiley, 2020) Hornschuh, Melanie; Zwicker, Paula; Schmidt, Thomas; Finke, Birgit; Kramer, Axel; Müller, GeraldAntimicrobial coating of implant material with poly(hexamethylene biguanide) hydrochloride (PHMB) may be an eligible method for preventing implant-associated infections. In the present study, an antibacterial effective amount of PHMB is adsorbed on the surface of titanium alloy after simple chemical pretreatment. Either oxidation with 5% H2O2 for 24 hr or processing for 2 hr in 5 M NaOH provides the base for the subsequent formation of a relatively stable self-assembled PHMB layer. Compared with an untreated control group, adsorbed PHMB produces no adverse effects on SaOs-2 cells within 48 hr cell culture, but promotes the initial attachment and spreading of the osteoblasts within 15 min. Specimens were inoculated with slime-producing bacteria to simulate a perioperative infection. Adsorbed PHMB reacts bactericidally against Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa after surface contact. Adhered SaOs-2 cells differentiate and produce alkaline phosphatase and deposit calcium within 4 days in a mineralization medium on PHMB-coated Ti6Al4V surfaces, which have been precontaminated with S. epidermidis. The presented procedures provide a simple method for generating biocompatibly and antimicrobially effective implant surfaces that may be clinically important. © 2019 The Authors. Journal of Biomedical Materials Research Part B: Applied Biomaterials published by Wiley Periodicals, Inc.
- ItemFirst‐mode of negative streamers: Inception at liquid/solid interfaces(Hoboken, NJ : Wiley, 2021) Ariza, David; Beroual, Abderrahmane; Methling, Ralf; Gortschakow, Sergey; Chamorro, Harold R.An experimental study of the inception of the first-mode negative streamer at liquid/solid interfaces is presented in this article. The study is performed with a point-plane configuration under square high voltage pulses. The electrode configuration is immersed in mineral oil and the liquid/solid interface is assembled in contact with the point electrode or in its vicinity. Four polymers and two impregnated papers have been tested as solids of the liquid/solid interface. Thus, it is possible to compare the influence of different parameter of the solid and the interface on the streamer inception. For example: Permittivity, solid surface roughness, chemical composition, etc. It has been observed that streamer inception voltages at interfaces with solids of higher permittivity to that of the mineral oil are statistically similar. Additionally, streamer inception voltages of streamer initiated free in the oil (no liquid/solid interface) are similar to that of the inception voltage of cases with solids with high permittivity. In contrast, the inception voltage of streamers initiated at permittivity matched interfaces are shown to be highest of the cases. The streamer inception voltage is also studied for different distances between the liquid/solid interface and the point electrode with a permittivity matched interface. The results show a dependency of the inception voltage and the distance between the point electrode and the interface. Finally, an analysis of the observation is performed to show that the Townsend-Meek criterion cannot predict the obtained results.