Search Results
Reconstruction of Ultra-thin Alveolar-capillary Basement Membrane Mimics
Alveolar-capillary basement membrane (BM) is ultra-thin (<2 µm) extracellular matrix that maintains integral epithelial-endothelial cell layers. In vitro reconstructions of alveolar-capillary barrier supported on synthetic scaffolds closely resembling the fibrous and ultra-thin natural BM are essential in mimicking the lung pathophysiology. Although BM topology and dimensions are well known to significantly influence cellular behavior, conventionally used BM mimics fail to recreate this natural niche. To overcome this, electrospun ultra-thin 2 µm poly(caprolactone) (PCL) nanofibrous mesh is used to establish an alveolar-capillary barrier model of lung endothelial/epithelial cells. Transepithelial electrical resistance (TEER) and permeability studies reveal integral tight junctions and improved mass transport through the highly porous PCL meshes compared to conventional dense membranes with etched pores. The chemotaxis of neutrophils is shown across the barrier in presence of inflammatory response that is naturally impeded in confined regions. Conventional requirement of 3 µm or larger pore size can lead to barrier disruption due to epithelial/endothelial cell invasion. Despite high porosity, the interconnected BM mimic prevents barrier disruption and allows neutrophil transmigration, thereby demonstrating the physiological relevance of the thin nanofibrous meshes. It is envisioned that these bipolar cultured barriers would contribute to an organ-level in vitro model for pathological disease, environmental pollutants, and nanotoxicology. © 2021 The Authors. Advanced Biology published by Wiley-VCH GmbH
Compiling geophysical and geological information into a 3-D model of the glacially-affected island of Föhr
Within the scope of climatic change and associated sea level rise, coastal aquifers are endangered and are becoming more a focus of research to ensure the future water supply in coastal areas. For groundwater modelling a good understanding of the geological/hydrogeological situation and the aquifer behavior is necessary. In preparation of groundwater modelling and assessment of climate change impacts on coastal water resources, we setup a geological/hydrogeological model for the North Sea Island of Föhr. Data from different geophysical methods applied from the air, the surface and in boreholes contribute to the 3-D model, e.g. airborne electromagnetics (SkyTEM) for spatial mapping the resistivity of the entire island, seismic reflections for detailed cross-sections in the groundwater catchment area, and geophysical borehole logging for calibration of these measurements. An iterative and integrated evaluation of the results from the different geophysical methods contributes to reliable data as input for the 3-D model covering the whole island and not just the well fields. The complex subsurface structure of the island is revealed. The local waterworks use a freshwater body embedded in saline groundwater. Several glaciations reordered the youngest Tertiary and Quaternary sediments by glaciotectonic thrust faulting, as well as incision and refill of glacial valleys. Both subsurface structures have a strong impact on the distribution of freshwater-bearing aquifers. A digital geological 3-D model reproduces the hydrogeological structure of the island as a base for a groundwater model. In the course of the data interpretation, we deliver a basis for rock identification. We demonstrate that geophysical investigation provide petrophysical parameters and improve the understanding of the subsurface and the groundwater system. The main benefit of our work is that the successful combination of electromagnetic, seismic and borehole data reveals the complex geology of a glacially-affected island. A sound understanding of the subsurface structure and the compilation of a 3-D model is imperative and the basis for a groundwater flow model to predict climate change effects on future water resources.
Semantic annotation for 3D cultural artefacts: MVP
A suite of tools for semantic annotation of 3D cultural artefacts is being developed as part of the NFDI4Culture project across several partner organisations (led by the Open Science lab at TIB, Hannover). Operating within Task area 1: Data capture and enrichment, the proposed toolchain focuses on the annotation of 3D data within a knowledge graph environment, so that 3D objects’ geometry, attendant metadata, as well as annotations remain searchable, while data interconnections are not lost.