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A versatile and customizable low-cost 3D-printed open standard for microscopic imaging

2020, Diederich, Benedict, Lachmann, René, Carlstedt, Swen, Marsikova, Barbora, Wang, Haoran, Uwurukundo, Xavier, Mosig, Alexander S., Heintzmann, Rainer

Modern microscopes used for biological imaging often present themselves as black boxes whose precise operating principle remains unknown, and whose optical resolution and price seem to be in inverse proportion to each other. With UC2 (You. See. Too.) we present a low-cost, 3D-printed, open-source, modular microscopy toolbox and demonstrate its versatility by realizing a complete microscope development cycle from concept to experimental phase. The self-contained incubator-enclosed brightfield microscope monitors monocyte to macrophage cell differentiation for seven days at cellular resolution level (e.g. 2 μm). Furthermore, by including very few additional components, the geometry is transferred into a 400 Euro light sheet fluorescence microscope for volumetric observations of a transgenic Zebrafish expressing green fluorescent protein (GFP). With this, we aim to establish an open standard in optics to facilitate interfacing with various complementary platforms. By making the content and comprehensive documentation publicly available, the systems presented here lend themselves to easy and straightforward replications, modifications, and extensions.

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Assessment of Advanced Oxidation Processes Using Zebrafish in a Non-Forced Exposure System: A Proof of Concept

2021, Cabascango, Tamia, Ortiz, Karol, Sandoval Pauker, Christian, Espinoza Pavón, Isabel, Ramoji, Anuradha, Popp, Jürgen, Pérez, Jady, Pinto, C. Miguel, Rivera-Parra, José Luis, Muñoz-Bisesti, Florinella, Aldás, María Belén, Araújo, Cristiano V. M., Vargas Jentzsch, Paul

Water bodies and aquatic ecosystems are threatened by discharges of industrial waters. Ecotoxicological effects of components occurring in untreated and treated wastewaters are often not considered. The use of a linear, multi-compartmented, non-forced, static system constructed with PET bottles is proposed for the quality assessment of treated waters, to deal with such limitations. Two synthetic waters, one simulating wastewater from the textile industry and the other one simulating wastewater from the cassava starch industry, were prepared and treated by homogeneous Fenton process and heterogeneous photocatalysis, respectively. Untreated and treated synthetic waters and their dilutions were placed into compartments of the non-forced exposure system, in which zebrafish (Danio rerio), the indicator organism, could select the environment of its preference. Basic physical–chemical and chemical parameters of untreated and treated synthetic waters were measured. The preference and avoidance responses allowed verification of whether or not the quality of the water was improved due to the treatment. The results of these assays can be a complement to conventional parameters of water quality.