Search Results
Polymer Brush-Functionalized Chitosan Hydrogels as Antifouling Implant Coatings
2017, Buzzacchera, Irene, Vorobii, Mariia, Kostina, Nina Yu, de Los Santos Pereira, Andres, Riedel, Tomáš, Bruns, Michael, Ogieglo, Wojciech, Möller, Martin, Wilson, Christopher J., Rodriguez-Emmenegger, Cesar
Implantable sensor devices require coatings that efficiently interface with the tissue environment to mediate biochemical analysis. In this regard, bioinspired polymer hydrogels offer an attractive and abundant source of coating materials. However, upon implantation these materials generally elicit inflammation and the foreign body reaction as a consequence of protein fouling on their surface and concomitant poor hemocompatibility. In this report we investigate a strategy to endow chitosan hydrogel coatings with antifouling properties by the grafting of polymer brushes in a "grafting-from" approach. Chitosan coatings were functionalized with polymer brushes of oligo(ethylene glycol) methyl ether methacrylate and 2-hydroxyethyl methacrylate using photoinduced single electron transfer living radical polymerization and the surfaces were thoroughly characterized by XPS, AFM, water contact angle goniometry, and in situ ellipsometry. The antifouling properties of these new bioinspired hydrogel-brush coatings were investigated by surface plasmon resonance. The influence of the modifications to the chitosan on hemocompatibility was assessed by contacting the surfaces with platelets and leukocytes. The coatings were hydrophilic and reached a thickness of up to 180 nm within 30 min of polymerization. The functionalization of the surface with polymer brushes significantly reduced the protein fouling and eliminated platelet activation and leukocyte adhesion. This methodology offers a facile route to functionalizing implantable sensor systems with antifouling coatings that improve hemocompatibility and pave the way for enhanced device integration in tissue.
Photodissociation of aligned CH3I and C6H3F2I molecules probed with time-resolved Coulomb explosion imaging by site-selective extreme ultraviolet ionization
2018, Amini, Kasra, Savelyev, Evgeny, Brauße, Felix, Berrah, Nora, Bomme, Cédric, Brouard, Mark, Burt, Michael, Christensen, Lauge, Düsterer, Stefan, Erk, Benjamin, Höppner, Hauke, Kierspel, Thomas, Krecinic, Faruk, Lauer, Alexandra, Lee, Jason W. L., Müller, Maria, Müller, Erland, Mullins, Terence, Redlin, Harald, Schirmel, Nora, Thøgersen, Jan, Techert, Simone, Toleikis, Sven, Treusch, Rolf, Trippel, Sebastian, Ulmer, Anatoli, Vallance, Claire, Wiese, Joss, Johnsson, Per, Küpper, Jochen, Rudenko, Artem, Rouzée, Arnaud, Stapelfeldt, Henrik, Rolles, Daniel, Boll, Rebecca
We explore time-resolved Coulomb explosion induced by intense, extreme ultraviolet (XUV) femtosecond pulses from a free-electron laser as a method to image photo-induced molecular dynamics in two molecules, iodomethane and 2,6-difluoroiodobenzene. At an excitation wavelength of 267 nm, the dominant reaction pathway in both molecules is neutral dissociation via cleavage of the carbon-iodine bond. This allows investigating the influence of the molecular environment on the absorption of an intense, femtosecond XUV pulse and the subsequent Coulomb explosion process. We find that the XUV probe pulse induces local inner-shell ionization of atomic iodine in dissociating iodomethane, in contrast to non-selective ionization of all photofragments in difluoroiodobenzene. The results reveal evidence of electron transfer from methyl and phenyl moieties to a multiply charged iodine ion. In addition, indications for ultrafast charge rearrangement on the phenyl radical are found, suggesting that time-resolved Coulomb explosion imaging is sensitive to the localization of charge in extended molecules.
Grafting of functional methacrylate polymer brushes by photoinduced SET-LRP
2016, Vorobii, Mariia, Pop-Georgievski, Ognen, de los Santos Pereira, Andres, Kostina, Nina Yu., Jezorek, Ryan, Sedláková, Zdeňka, Percec, Virgil, Rodriguez-Emmenegger, Cesar
Photoinduced surface-initiated single electron transfer living radical polymerization (SET-LRP) is a versatile technique for the preparation of polymer brushes. The vast diversity of compatible functional groups, together with a high end-group fidelity that enables precise control of the architecture, makes this approach an effective tool for tuning the properties of surfaces. We report the application of photoinduced SET-LRP for the surface-initiated grafting of polymer brushes from a wide range of methacrylate monomers for the first time. The living character of the process was demonstrated by the linear evolution of the polymer brush thickness in time, the ability to reinitiate the polymerization for the preparation of well-defined block copolymers, and also by X-ray photoelectron spectroscopy depth profiling. The surface patterning with these brushes could be achieved simply by restricting the irradiated area. The ability of poly(methacrylate) brushes prepared in this way to prevent non-specific protein adsorption is also demonstrated, indicating the suitability of this procedure for advanced applications.
Electron-transfer initiated nucleophilic substitution of thiophenolate anion by 1-chloro-substituted 4-(thiazol-2-ylazo)naphthalenes
2020, Dmitrieva, E., Yu, X., Hartmann, H.
In this work, the electrochemical transformation of 5-chloro-2-[(4-chloronaphthalen-1-yl)azo]thiazoles (A) into the corresponding radical anion A·− and its subsequent reaction with diphenyldisulfide (PhSSPh) was studied. It was found that the primarily generated azo anion radical A·− is able to initiate an electron transfer process which converts the disulfide into its thiolate anion PhS−. This anion was subsequently able to substitute the Cl- and H-groups by phenylmercapto moieties in the starting azo compound A. The structures of the phenylmercapto-substituted azo compounds thus generated were confirmed by thin-layer chromatography and mass spectrometry using independently prepared compounds as references.