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- ItemDiscrimination between pathogenic and non-pathogenic E. coli strains by means of Raman microspectroscopy(Berlin ; Heidelberg : Springer, 2020) Lorenz B.; Ali N.; Bocklitz T.; Rösch P.; Popp J.Bacteria can be harmless commensals, beneficial probiotics, or harmful pathogens. Therefore, mankind is challenged to detect and identify bacteria in order to prevent or treat bacterial infections. Examples are identification of species for treatment of infection in clinics and E. coli cell counting for water quality monitoring. Finally, in some instances, the pathogenicity of a species is of interest. The main strategies to investigate pathogenicity are detection of target genes which encode virulence factors. Another strategy could be based on phenotypic identification. Raman spectroscopy is a promising phenotypic method, which offers high sensitivities and specificities for the identification of bacteria species. In this study, we evaluated whether Raman microspectroscopy could be used to determine the pathogenicity of E. coli strains. We used Raman spectra of seven non-pathogenic and seven pathogenic E. coli strains to train a PCA-SVM model. Then, the obtained model was tested by identifying the pathogenicity of three additional E. coli strains. The pathogenicity of these three strains could be correctly identified with a mean sensitivity of 77%, which is suitable for a fast screening of pathogenicity of single bacterial cells. [Figure not available: see fulltext.]. © 2020, The Author(s).
- ItemCharacterization of a regional coastal zone aquifer using an interdisciplinary approach – an example from Weser-Elbe region, Lower Saxony, Germany(Les Ulis : EDP Sciences, 2018) Rahman, Mohammad Azizur; González, Eva; Wiederhold, Helga; Deus, Nico; Elbracht, Jörg; Siemon, Bernhard; Szymkiewicz, Adam; Sadurski, A.; Jaworska-Szulc, B.In this study, interdisciplinary approaches are considered to characterize the coastal zone aquifer of the Elbe-Weser region in the North of Lower Saxony, Germany. Geological, hydrogeological, geochemical and geophysical information have been considered to analyze the current status of the aquifers. All the information collectively states that the salinity distribution in the subsurface is heterogeneous both horizontally and vertically. Early age flooding also contributed to this heterogeneity. No general classification of groundwater quality (according to some piper diagrams) could be identified. Helicopter-borne electro-magnetic data clearly show the presence of freshwater reserves below the sea near the west coast. Groundwater recharge largely happens in the moraine ridges (west side of the area) where both the surface elevation and the groundwater level are high. Consequently, submarine groundwater discharge occurs from the same place. All these information will facilitate to develop the planned density driven groundwater flow and transport model for the study area.
- ItemHydrological extremes and security(Göttingen : Copernicus GmbH, 2015) Kundzewicz, Z.W.; Matczak, P.
- ItemReal-time stimulated Raman spectroscopy with a non-collinear optical parametric oscillator(Washington, DC : Soc., 2021) Beichert, Luise; Binhammer, Yuliya; Andrade, José R. C.; Mevert, Robin; Kniggendorf, Ann-Kathrin; Roth, Bernhard; Morgner, UweUltrafast detection of microplastic particles is becoming a vital problem, as these particles are found in water sources worldwide. Ideally, a live analysis in flow is desirable to directly monitor the water quality for contaminations. Therefore, coherent Raman spectroscopy techniques require fast and broadband tunable lasers to address all relevant spectral regions of the investigated samples. In our work, we combine a high power non-collinear optical parametric oscillator with a real-time stimulated Raman scattering spectroscopy setup. The light source is continously tunable from 700 nm to 1030 nm in less than 10 ms, delivering an average output power of more than 500 mW with sub-ps pulses. We show the immediate observation of mixing processes and the detection of microplastic particles in water solution with a spectral window of more than 2000 cm-1.
- ItemEffect of climate change on hydrology, sediment and nutrient losses in two lowland catchments in Poland(Basel : MDPI AG, 2017) Marcinkowski, P.; Piniewski, M.; Kardel, I.; Szcześniak, M.; Benestad, R.; Srinivasan, R.; Ignar, S.; Okruszko, T.Future climate change is projected to have significant impact on water resources availability and quality in many parts of the world. The objective of this paper is to assess the effect of projected climate change on water quantity and quality in two lowland catchments (the Upper Narew and the Barycz) in Poland in two future periods (near future: 2021-2050, and far future: 2071-2100). The hydrological model SWAT was driven by climate forcing data from an ensemble of nine bias-corrected General Circulation Models-Regional Climate Models (GCM-RCM) runs based on the Coordinated Downscaling Experiment-European Domain (EURO-CORDEX). Hydrological response to climate warming and wetter conditions (particularly in winter and spring) in both catchments includes: lower snowmelt, increased percolation and baseflow and higher runoff. Seasonal differences in the response between catchments can be explained by their properties (e.g., different thermal conditions and soil permeability). Projections suggest only moderate increases in sediment loss, occurring mainly in summer and winter. A sharper increase is projected in both catchments for TN losses, especially in the Barycz catchment characterized by a more intensive agriculture. The signal of change in annual TP losses is blurred by climate model uncertainty in the Barycz catchment, whereas a weak and uncertain increase is projected in the Upper Narew catchment.
- ItemModeling climate and management change impacts on water quality and in-stream processes in the Elbe river basin(Basel : MDPI AG, 2016) Hesse, C.; Krysanova, V.