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Subject: article Ă— Subject: microfluidic analysis Ă—

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    Real-time image processing for label-free enrichment of Actinobacteria cultivated in picolitre droplets
    (London [u.a.] : Royal Society of Chemistry, 2013) Zang, E.; Brandes, S.; Tovar, M.; Martin, K.; Mech, F.; Horbert, P.; Henkel, T.; Figge, M.T.; Roth, M.
    The majority of today's antimicrobial therapeutics is derived from secondary metabolites produced by Actinobacteria. While it is generally assumed that less than 1% of Actinobacteria species from soil habitats have been cultivated so far, classic screening approaches fail to supply new substances, often due to limited throughput and frequent rediscovery of already known strains. To overcome these restrictions, we implement high-throughput cultivation of soil-derived Actinobacteria in microfluidic pL-droplets by generating more than 600000 pure cultures per hour from a spore suspension that can subsequently be incubated for days to weeks. Moreover, we introduce triggered imaging with real-time image-based droplet classification as a novel universal method for pL-droplet sorting. Growth-dependent droplet sorting at frequencies above 100 Hz is performed for label-free enrichment and extraction of microcultures. The combination of both cultivation of Actinobacteria in pL-droplets and real-time detection of growing Actinobacteria has great potential in screening for yet unknown species as well as their undiscovered natural products.
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    Trapping self-propelled micromotors with microfabricated chevron and heart-shaped chips
    (Cambridge : Royal Society of Chemistry, 2014) Restrepo-PĂ©rez, Laura; Soler, LluĂ­s; MartĂ­nez-Cisneros, Cynthia S.; Sanchez, Samuel; Schmidt, Oliver G.
    We demonstrate that catalytic micromotors can be trapped in microfluidic chips containing chevron and heart-shaped structures. Despite the challenge presented by the reduced size of the traps, microfluidic chips with different trapping geometries can be fabricated via replica moulding. We prove that these microfluidic chips can capture micromotors without the need for any external mechanism to control their motion.
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    Biofunctionalized self-propelled micromotors as an alternative on-chip concentrating system
    (Cambridge : Royal Society of Chemistry, 2014) Restrepo-PĂ©rez, Laura; Meyer, Anne K.; Helbig, Linda; Sanchez, Samuel; Schmidt, Oliver G.
    Sample pre-concentration is crucial to achieve high sensitivity and low detection limits in lab-on-a-chip devices. Here, we present a system in which self-propelled catalytic micromotors are biofunctionalized and trapped acting as an alternative concentrating mechanism. This system requires no external energy source, which facilitates integration and miniaturization.
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    Magnetoresistive emulsion analyzer
    (London : Nature Publishing Group, 2013) Lin, G.; Baraban, L.; Han, L.; Karnaushenko, D.; Makarov, D.; Cuniberti, G.; Schmidt, O.G.
    We realize a magnetoresistive emulsion analyzer capable of detection, multiparametric analysis and sorting of ferrofluid-containing nanoliter-droplets. The operation of the device in a cytometric mode provides high throughput and quantitative information about the dimensions and magnetic content of the emulsion. Our method offers important complementarity to conventional optical approaches involving ferrofluids, and paves the way to the development of novel compact tools for diagnostics and nanomedicine including drug design and screening.