2011, Rupf, S., Idlibi, A.N., Marrawi, F.A., Hannig, M., Schubert, A., von Mueller, L., Spitzer, W., Holtmann, H., Lehmann, A., Rueppell, A., Schindler, A.

The removal of biofilms from microstructured titanium used for dental implants is a still unresolved challenge. This experimental study investigated disinfection and removal of in situ formed biofilms from microstructured titanium using cold atmospheric plasma in combination with air/water spray. Titanium discs (roughness (Ra): 1.96 μm) were exposed to human oral cavities for 24 and 72 hours (n = 149 each) to produce biofilms. Biofilm thickness was determined using confocal laser scanning microscopy (n = 5 each). Plasma treatment of biofilms was carried out ex vivo using a microwave-driven pulsed plasma source working at temperatures from 39 to 43°C. Following plasma treatment, one group was air/water spray treated before re-treatment by second plasma pulses. Vital microorganisms on the titanium surfaces were identified by contact culture (Rodac agar plates). Biofilm presence and bacterial viability were quantified by fluorescence microscopy. Morphology of titanium surfaces and attached biofilms was visualized by scanning electron microscopy (SEM). Total protein amounts of biofilms were colorimetrically quantified. Untreated and air/water treated biofilms served as controls. Cold plasma treatment of native biofilms with a mean thickness of 19 μm (24 h) to 91 μm (72 h) covering the microstructure of the titanium surface caused inactivation of biofilm bacteria and significant reduction of protein amounts. Total removal of biofilms, however, required additional application of air/water spray, and a second series of plasma treatment. Importantly, the microstructure of the titanium discs was not altered by plasma treatment. The combination of atmospheric plasma and non-abrasive air/water spray is applicable for complete elimination of oral biofilms from microstructured titanium used for dental implants and may enable new routes for the therapy of periimplant disease.

2016, Krauß, Michael, Drastig, Katrin, Prochnow, Annette, Rose-Meierhöfer, Sandra, Kraatz, Simone

Water is used in dairy farming for producing feed, watering the animals, and cleaning and disinfecting barns and equipment. The objective of this study was to investigate the drinking and cleaning water use in a dairy cow barn. The water use was measured on a well-managed commercial dairy farm in North-East Germany. Thirty-eight water meters were installed in a barn with 176 cows and two milking systems (an automatic milking system and a herringbone parlour). Their counts were logged hourly over 806 days. On average, the cows in the automatic milking system used 91.1 (SD 14.3) L drinking water per cow per day, while those in the herringbone parlour used 54.4 (SD 5.3) L per cow per day. The cows drink most of the water during the hours of (natural and artificial) light in the barn. Previously published regression functions of drinking water intake of the cows were reviewed and a new regression function based on the ambient temperature and the milk yield was developed (drinking water intake (L per cow per day) = −27.937 + 0.49 × mean temperature + 3.15 × milk yield (R2 = 0.67)). The cleaning water demand had a mean of 28.6 (SD 14.8) L per cow per day in the automatic milking system, and a mean of 33.8 (SD 14.1) L per cow per day in the herringbone parlour. These findings show that the total technical water use in the barn makes only a minor contribution to water use in dairy farming compared with the water use for feed production.