2 results
Search Results
Now showing 1 - 2 of 2
- ItemA novel, low-volume method for organ culture of embryonic kidneys that allows development of cortico-medullary anatomical organization(San Francisco, Calif. : Public Library of Science, 2010) Sebinger, D.D.R.; Unbekandt, M.; Ganeva, V.V.; Ofenbauer, A.; Werner, C.; Davies, J.A.Here, we present a novel method for culturing kidneys in low volumes of medium that offers more organotypic development compared to conventional methods. Organ culture is a powerful technique for studying renal development. It recapitulates many aspects of early development very well, but the established techniques have some disadvantages: in particular, they require relatively large volumes (1-3 mls) of culture medium, which can make high-throughput screens expensive, they require porous (filter) substrates which are difficult to modify chemically, and the organs produced do not achieve good cortico-medullary zonation. Here, we present a technique of growing kidney rudiments in very low volumes of medium-around 85 microliters-using silicone chambers. In this system, kidneys grow directly on glass, grow larger than in conventional culture and develop a clear anatomical cortico-medullary zonation with extended loops of Henle. © 2010 Sebinger et al.
- ItemUntersuchung neuartiger kombinierter Vernetzungsmechanismen für die Pulverlackhärtung unter Einfluss von Katalysatoren, Teilprojekt 1/Teil 1; Untersuchungen zur thermischen und katalysierten Spaltung von Uretdionen als verkappungsmittelfrei blockierte Isocyanate, Teilprojekt 1/Teil 2; Einfluss von Additiven und Pigmenten auf die Grenzflächenphänomene bei der Pulverlackbeschichtung, Teilprojekt 2/Teil 3 : Abschlussbericht Teil 1 - 3 zum Teilprojekt 1 - 2 im Rahmen des F und E-Programmes zur Erweiterung der Anwendung der Pulverlackiertechnik(Dresden : Leibniz-Institut für Polymerforschung, 2000) Gedan-Smolka, Michaela; Meier Haack, Jochen; Lehmann, Frank; Grundke, Karina; Uhlmann, Petra; Wulf, Martin[no abstract available]