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Title: Melt Electrowriting of Scaffolds with a Porosity Gradient to Mimic the Matrix Structure of the Human Trabecular Meshwork
Authors: Włodarczyk-Biegun, Małgorzata K.Villiou, MariaKoch, MarcusMuth, ChristinaWang, PeixiOtt, Jennadel Campo, Aranzazu
Publishers version: https://doi.org/10.1101/2022.05.28.476655
URI: https://oa.tib.eu/renate/handle/123456789/11493
http://dx.doi.org/10.34657/10527
Issue Date: 2022
Published in: bioRxiv
Journal: bioRxiv
Publisher: New York : Cold Spring Harbor Laboratory
Abstract: The permeability of the Human Trabecular Meshwork (HTM) regulates eye pressure via a porosity gradient across its thickness modulated by stacked layers of matrix fibrils and cells. Changes in HTM porosity are associated with increases in intraocular pressure and the progress of diseases like glaucoma. Engineered HTMs could help to understand the structure-function relation in natural tissues, and lead to new regenerative solutions. Here, melt electrowriting (MEW) is explored as a biofabrication technique to produce fibrillar, porous scaffolds that mimic the multilayer, gradient structure of native HTM. Poly(caprolactone) constructs with a height of 125-500 μm and fiber diameters of 10-12 μm are printed. Scaffolds with a tensile modulus between 5.6 and 13 MPa, and a static compression modulus in the range of 6-360 kPa are obtained by varying the scaffolds design, i.e., density and orientation of the fibers and number of stacked layers. Primary HTM cells attach to the scaffolds, proliferate, and form a confluent layer within 8-14 days, depending on the scaffold design. High cell viability and cell morphology close to that in the native tissue are observed. The present work demonstrates the utility of MEW to reconstruct complex morphological features of natural tissues.
Keywords: melt electrowriting; human trabecular meshwork; glaucoma; 3D printing; poly(caprolactone); tissue engineering
Type: workingPaper; Text
Publishing status: draft
DDC: 570
License: CC BY 4.0 Unported
Link to license: https://creativecommons.org/licenses/by/4.0/
Appears in Collections:Biowissenschaften

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Włodarczyk-Biegun, Małgorzata K., Maria Villiou, Marcus Koch, Christina Muth, Peixi Wang, Jenna Ott and Aranzazu del Campo, 2022. Melt Electrowriting of Scaffolds with a Porosity Gradient to Mimic the Matrix Structure of the Human Trabecular Meshwork. 2022. New York : Cold Spring Harbor Laboratory
Włodarczyk-Biegun, M. K., Villiou, M., Koch, M., Muth, C., Wang, P., Ott, J. and del Campo, A. (2022) “Melt Electrowriting of Scaffolds with a Porosity Gradient to Mimic the Matrix Structure of the Human Trabecular Meshwork.” New York : Cold Spring Harbor Laboratory. doi: https://doi.org/10.1101/2022.05.28.476655.
Włodarczyk-Biegun M K, Villiou M, Koch M, Muth C, Wang P, Ott J, del Campo A. Melt Electrowriting of Scaffolds with a Porosity Gradient to Mimic the Matrix Structure of the Human Trabecular Meshwork. New York : Cold Spring Harbor Laboratory; 2022.
Włodarczyk-Biegun, M. K., Villiou, M., Koch, M., Muth, C., Wang, P., Ott, J., & del Campo, A. (2022). Melt Electrowriting of Scaffolds with a Porosity Gradient to Mimic the Matrix Structure of the Human Trabecular Meshwork (Version draft). Version draft. New York : Cold Spring Harbor Laboratory. https://doi.org/https://doi.org/10.1101/2022.05.28.476655
Włodarczyk-Biegun M K, Villiou M, Koch M, Muth C, Wang P, Ott J, del Campo A. Melt Electrowriting of Scaffolds with a Porosity Gradient to Mimic the Matrix Structure of the Human Trabecular Meshwork. Published online 2022. doi:https://doi.org/10.1101/2022.05.28.476655


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