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Title: | Red blood cell passage of small capillaries is associated with transient Ca2+-mediated adaptations |
Authors: | Danielczok, Jens G.; Terriac, Emmanuel; Hertz, Laura; Petkova-Kirova, Polina; Lautenschläger, Franziska; Laschke, Matthias W.; Kaestner, Lars |
Publishers version: | https://doi.org/10.3389/fphys.2017.00979 |
URI: | https://doi.org/10.34657/444 https://oa.tib.eu/renate/handle/123456789/4627 |
Issue Date: | 2017 |
Published in: | Frontiers in Physiology, Volume 8 |
Journal: | Frontiers in Physiology |
Volume: | 8 |
Publisher: | Lausanne : Frontiers Media |
Abstract: | When red blood cells (RBCs) pass constrictions or small capillaries they need to pass apertures falling well below their own cross section size. We used different means of mechanical stimulations (hypoosmotic swelling, local mechanical stimulation, passing through microfluidic constrictions) to observe cellular responses of human RBCs in terms of intracellular Ca2+-signaling by confocal microscopy of Fluo-4 loaded RBCs.We were able to confirm ourin vitro results in a mouse dorsal skinfold chamber model showing a transiently increased intracellular Ca2+ when RBCs were passing through small capillaries in vivo. Furthermore, we performed the above-mentioned in vitro experiments as well as measurements of RBCs filterability under various pharmacological manipulations (GsMTx-4, TRAM-34) to explore the molecular mechanism of the Ca2+-signaling. Based on these experiments we conclude that mechanical stimulation of RBCs activates mechano-sensitive channels most likely Piezo1. This channel activity allows Ca2+ to enter the cell, leading to a transient activation of the Gardos-channel associated with K+, Cl−, and water loss, i.e., with a transient volume adaptation facilitating the passage of the RBCs through the constricti on. |
Keywords: | RBC deformation; Piezo1; hSK4 (KCNN4); Ca2+ imaging; microfluidics; dorsal skinfold chamber |
Type: | article; Text |
Publishing status: | publishedVersion |
DDC: | 570 |
License: | Dieses Dokument darf im Rahmen von § 53 UrhG zum eigenen Gebrauch kostenfrei heruntergeladen, gelesen, gespeichert und ausgedruckt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. This document may be downloaded, read, stored and printed for your own use within the limits of § 53 UrhG but it may not be distributed via the internet or passed on to external parties. |
Appears in Collections: | Biowissenschaften |
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Danielczok, Jens G., Emmanuel Terriac, Laura Hertz, Polina Petkova-Kirova, Franziska Lautenschläger, Matthias W. Laschke and Lars Kaestner, 2017. Red blood cell passage of small capillaries is associated with transient Ca2+-mediated adaptations. 2017. Lausanne : Frontiers Media
Danielczok, J. G., Terriac, E., Hertz, L., Petkova-Kirova, P., Lautenschläger, F., Laschke, M. W. and Kaestner, L. (2017) “Red blood cell passage of small capillaries is associated with transient Ca2+-mediated adaptations.” Lausanne : Frontiers Media. doi: https://doi.org/10.3389/fphys.2017.00979.
Danielczok J G, Terriac E, Hertz L, Petkova-Kirova P, Lautenschläger F, Laschke M W, Kaestner L. Red blood cell passage of small capillaries is associated with transient Ca2+-mediated adaptations. Vol. 8. Lausanne : Frontiers Media; 2017.
Danielczok, J. G., Terriac, E., Hertz, L., Petkova-Kirova, P., Lautenschläger, F., Laschke, M. W., & Kaestner, L. (2017). Red blood cell passage of small capillaries is associated with transient Ca2+-mediated adaptations (Version publishedVersion, Vol. 8). Version publishedVersion, Vol. 8. Lausanne : Frontiers Media. https://doi.org/https://doi.org/10.3389/fphys.2017.00979
Danielczok J G, Terriac E, Hertz L, Petkova-Kirova P, Lautenschläger F, Laschke M W, Kaestner L. Red blood cell passage of small capillaries is associated with transient Ca2+-mediated adaptations. 2017;8. doi:https://doi.org/10.3389/fphys.2017.00979
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