Please use this identifier to cite or link to this item: https://oa.tib.eu/renate/handle/123456789/6052
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dc.rights.licenseCC BY-NC-ND 4.0 Unportedeng
dc.contributor.authorNair, Roshna V.-
dc.contributor.authorZhao, Shifang-
dc.contributor.authorTerriac, Emmanuel-
dc.contributor.authorLautenschläger, Franziska-
dc.contributor.authorHetmanski, Joseph H.R.-
dc.contributor.authorCaswell, Patrick T.-
dc.contributor.authordel Campo, Aranzazu-
dc.date.accessioned2021-01-06T11:40:32Z-
dc.date.available2021-01-06T11:40:32Z-
dc.date.issued2020-
dc.identifier.urihttps://doi.org/10.34657/4681-
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/6052
dc.description.abstractThe study of the actin cytoskeleton and related cellular processes requires tools to specifically interfere with actin dynamics in living cell cultures, ideally with spatiotemporal control and compatible with real time imaging. A phototriggerable derivative of the actin disruptor Cytochalasin D (CytoD) is described and tested here. It includes a nitroveratryloxycarbonyl (Nvoc) photoremovable protecting group (PPG) at the hydroxyl group at C7 of CytoD. The attachment of the PPG renders Nvoc-CytoD temporarily inactive, and enables light-dosed delivery of the active drug CytoD to living cells. This article presents the full structural and physicochemical characterization, the toxicity analysis. It is complemented with biological tests to show the time scales (seconds) and spatial resolution (cellular level) achievable with a UV source in a regular microscopy setupeng
dc.language.isoengeng
dc.publisherWashington, D.C. : American Chemical Societyeng
dc.relation.ispartofseriesChemRxiveng
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/eng
dc.subjectpreprinteng
dc.subjectNvoc-CytoDeng
dc.subjectCytochalasin Deng
dc.subject.ddc540eng
dc.titlePossibilities and Limitations of Photoactivatable Cytochalasin D for the Spatiotemporal Regulation of Actin Dynamicseng
dc.typearticleeng
dc.typeTexteng
dc.description.versionsubmittedVersioneng
wgl.contributorINMeng
wgl.subjectChemieeng
wgl.typeZeitschriftenartikeleng
dc.relation.doihttps://doi.org/10.26434/chemrxiv.12609545.v1-
dcterms.bibliographicCitation.journalTitleChemRxiveng
tib.accessRightsopenAccesseng
Appears in Collections:Chemie

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Nair, Roshna V., Shifang Zhao, Emmanuel Terriac, Franziska Lautenschläger, Joseph H.R. Hetmanski, Patrick T. Caswell and Aranzazu del Campo, 2020. Possibilities and Limitations of Photoactivatable Cytochalasin D for the Spatiotemporal Regulation of Actin Dynamics. 2020. Washington, D.C. : American Chemical Society
Nair, R. V., Zhao, S., Terriac, E., Lautenschläger, F., Hetmanski, J. H. R., Caswell, P. T. and del Campo, A. (2020) “Possibilities and Limitations of Photoactivatable Cytochalasin D for the Spatiotemporal Regulation of Actin Dynamics.” Washington, D.C. : American Chemical Society. doi: https://doi.org/10.26434/chemrxiv.12609545.v1.
Nair R V, Zhao S, Terriac E, Lautenschläger F, Hetmanski J H R, Caswell P T, del Campo A. Possibilities and Limitations of Photoactivatable Cytochalasin D for the Spatiotemporal Regulation of Actin Dynamics. Washington, D.C. : American Chemical Society; 2020.
Nair, R. V., Zhao, S., Terriac, E., Lautenschläger, F., Hetmanski, J. H. R., Caswell, P. T., & del Campo, A. (2020). Possibilities and Limitations of Photoactivatable Cytochalasin D for the Spatiotemporal Regulation of Actin Dynamics (Version submittedVersion). Version submittedVersion. Washington, D.C. : American Chemical Society. https://doi.org/https://doi.org/10.26434/chemrxiv.12609545.v1
Nair R V, Zhao S, Terriac E, Lautenschläger F, Hetmanski J H R, Caswell P T, del Campo A. Possibilities and Limitations of Photoactivatable Cytochalasin D for the Spatiotemporal Regulation of Actin Dynamics. Published online 2020. doi:https://doi.org/10.26434/chemrxiv.12609545.v1


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