Recombinant phage coated 1D Al2O3 nanostructures for controlling the adhesion and proliferation of endothelial cells

A novel synthesis of a nanostructured cell adhesive surface is investigated for future stent developments. One-dimensional (1D) Al2O3 nanostructures were prepared by chemical vapor deposition of a single source precursor. Afterwards, recombinant filamentous bacteriophages which display a short binding motif with a cell adhesive peptide (RGD) on p3 and p8 proteins were immobilized on these 1D Al2O3 nanostructures by a simple dip-coating process to study the cellular response of human endothelial EA hy.926. While the cell density decreased on as-deposited 1D Al2O3 nanostructures, we observed enhanced cell proliferation and cell-cell interaction on recombinant phage overcoated 1D Al2O3 nanostructures. The recombinant phage overcoating also supports an isotropic cell spreading rather than elongated cell morphology as we observed on as-deposited Al2O3 1D nanostructures.

Keywords

Bacteriophages, Cell Adhesion, Cell Proliferation, Endothelial Cells, Humans, Nanostructures, Surface Properties, Filamentous bacteriophage, adhesive agent, aluminum oxide, nanocoating, peptides and proteins, protein p3, protein p8, unclassified drug, nanomaterial, binding affinity, cell density, cell interaction, cell spreading, cell structure, cell viability, endothelium cell, human cell, immobilized cell, nonhuman, recombinant bacteriophage, synthesis, chemistry, drug effects

URI

https://oa.tib.eu/renate/handle/123456789/9652
https://doi.org/10.34657/8690

Citation

Lee, J., Jeon, H., Haidar, A., Abdul-Khaliq, H., Veith, M., Aktas, C., & Kim, Y. (2015). Recombinant phage coated 1D Al2O3 nanostructures for controlling the adhesion and proliferation of endothelial cells. 2015. https://doi.org//10.1155/2015/909807

Collections

Medizin
Biowissenschaften

License

CC BY 3.0 Unported

https://creativecommons.org/licenses/by/3.0/

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