How to minimize dye-induced perturbations while studying biomembrane structure and dynamics: PEG linkers as a rational alternative

dc.bibliographicCitation.firstPage2436
dc.bibliographicCitation.issue11
dc.bibliographicCitation.lastPage2445
dc.bibliographicCitation.volume1860
dc.contributor.authorMobarak, Edouard
dc.contributor.authorJavanainen, Matti
dc.contributor.authorKulig, Waldemar
dc.contributor.authorHonigmann, Alf
dc.contributor.authorSezgin, Erdinc
dc.contributor.authorAho, Noora
dc.contributor.authorEggeling, Christian
dc.contributor.authorRog, Tomasz
dc.contributor.authorVattulainen, Ilpo
dc.date.accessioned2023-01-19T09:43:51Z
dc.date.available2023-01-19T09:43:51Z
dc.date.issued2018
dc.description.abstractOrganic dye-tagged lipid analogs are essential for many fluorescence-based investigations of complex membrane structures, especially when using advanced microscopy approaches. However, lipid analogs may interfere with membrane structure and dynamics, and it is not obvious that the properties of lipid analogs would match those of non-labeled host lipids. In this work, we bridged atomistic simulations with super-resolution imaging experiments and biomimetic membranes to assess the performance of commonly used sphingomyelin-based lipid analogs. The objective was to compare, on equal footing, the relative strengths and weaknesses of acyl chain labeling, headgroup labeling, and labeling based on poly-ethyl-glycol (PEG) linkers in determining biomembrane properties. We observed that the most appropriate strategy to minimize dye-induced membrane perturbations and to allow consideration of Brownian-like diffusion in liquid-ordered membrane environments is to decouple the dye from a membrane by a PEG linker attached to a lipid headgroup. Yet, while the use of PEG linkers may sound a rational and even an obvious approach to explore membrane dynamics, the results also suggest that the dyes exploiting PEG linkers interfere with molecular interactions and their dynamics. Overall, the results highlight the great care needed when using fluorescent lipid analogs, in particular accurate controls.eng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/10929
dc.identifier.urihttp://dx.doi.org/10.34657/9955
dc.language.isoeng
dc.publisherAmsterdam : Elsevier
dc.relation.doihttps://doi.org/10.1016/j.bbamem.2018.07.003
dc.relation.essn1879-2642
dc.relation.ispartofseriesBiochimica et biophysica acta : BBA 1860 (2018), Nr. 11
dc.relation.issn0005-2736
dc.rights.licenseCC BY 4.0 Unported
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAtomistic simulationeng
dc.subjectFluorescent probeeng
dc.subjectLipid membraneeng
dc.subjectMolecular dynamics simulationeng
dc.subjectPEG linkereng
dc.subjectSuper-resolution microscopyeng
dc.subject.ddc570
dc.titleHow to minimize dye-induced perturbations while studying biomembrane structure and dynamics: PEG linkers as a rational alternativeeng
dc.typearticleeng
dc.typeTexteng
dcterms.bibliographicCitation.journalTitleBiochimica et biophysica acta : BBA
tib.accessRightsopenAccesseng
wgl.contributorIPHT
wgl.subjectBiowissenschaften/Biologieger
wgl.subjectChemieger
wgl.typeZeitschriftenartikelger
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