Structural insights into heme binding to IL-36α proinflammatory cytokine
dc.bibliographicCitation.firstPage | 16893 | eng |
dc.bibliographicCitation.journalTitle | Scientific Reports | eng |
dc.bibliographicCitation.lastPage | 2435 | eng |
dc.bibliographicCitation.volume | 9 | eng |
dc.contributor.author | Wißbrock, Amelie | |
dc.contributor.author | Goradia, Nishit | |
dc.contributor.author | Kumar, Amit | |
dc.contributor.author | Paul George, Ajay Abisheck | |
dc.contributor.author | Kühl, Toni | |
dc.contributor.author | Bellstedt, Peter | |
dc.contributor.author | Ramachandran, Ramadurai | |
dc.contributor.author | Hoffmann, Patrick | |
dc.contributor.author | Galler, Kerstin | |
dc.contributor.author | Popp, Jürgen | |
dc.contributor.author | Neugebauer, Ute | |
dc.contributor.author | Hampel, Kornelia | |
dc.contributor.author | Zimmermann, Bastian | |
dc.contributor.author | Adam, Susanne | |
dc.contributor.author | Wiendl, Maximilian | |
dc.contributor.author | Krönke, Gerhard | |
dc.contributor.author | Hamza, Iqbal | |
dc.contributor.author | Heinemann, Stefan H. | |
dc.contributor.author | Frey, Silke | |
dc.contributor.author | Hueber, Axel J. | |
dc.contributor.author | Ohlenschläger, Oliver | |
dc.contributor.author | Imhof, Diana | |
dc.date.accessioned | 2020-01-03T14:03:31Z | |
dc.date.available | 2020-01-03T14:03:31Z | |
dc.date.issued | 2019 | |
dc.description.abstract | Cytokines of the interleukin (IL)-1 family regulate immune and inflammatory responses. The recently discovered IL-36 family members are involved in psoriasis, rheumatoid arthritis, and pulmonary diseases. Here, we show that IL-36α interacts with heme thereby contributing to its regulation. Based on in-depth spectroscopic analyses, we describe two heme-binding sites in IL-36α that associate with heme in a pentacoordinated fashion. Solution NMR analysis reveals structural features of IL-36α and its complex with heme. Structural investigation of a truncated IL-36α supports the notion that the N-terminus is necessary for association with its cognate receptor. Consistent with our structural studies, IL-36-mediated signal transduction was negatively regulated by heme in synovial fibroblast-like synoviocytes from rheumatoid arthritis patients. Taken together, our results provide a structural framework for heme-binding proteins and add IL-1 cytokines to the group of potentially heme-regulated proteins. | eng |
dc.description.version | publishedVersion | eng |
dc.identifier.uri | https://doi.org/10.34657/85 | |
dc.identifier.uri | https://oa.tib.eu/renate/handle/123456789/4814 | |
dc.language.iso | eng | eng |
dc.publisher | Berlin : Nature Publishing | eng |
dc.relation.doi | https://doi.org/10.1038/s41598-019-53231-0 | |
dc.rights.license | CC BY 4.0 Unported | eng |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | eng |
dc.subject.ddc | 610 | eng |
dc.subject.other | interleukin | eng |
dc.subject.other | IL-36 | eng |
dc.subject.other | inflammatory response | eng |
dc.title | Structural insights into heme binding to IL-36α proinflammatory cytokine | eng |
dc.type | Article | eng |
dc.type | Text | eng |
tib.accessRights | openAccess | eng |
wgl.contributor | IPHT | eng |
wgl.subject | Ingenieurwissenschaften | eng |
wgl.type | Zeitschriftenartikel | eng |
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