Distinction of nucleobases - A tip-enhanced Raman approach

dc.bibliographicCitation.firstPage628eng
dc.bibliographicCitation.issue1eng
dc.bibliographicCitation.volume2eng
dc.contributor.authorTreffer, R.
dc.contributor.authorLin, X.
dc.contributor.authorBailo, E.
dc.contributor.authorDeckert-Gaudig, T.
dc.contributor.authorDeckert, V.
dc.date.accessioned2020-09-11T12:53:01Z
dc.date.available2020-09-11T12:53:01Z
dc.date.issued2011
dc.description.abstractThe development of novel DNA sequencing methods is one of the ongoing challenges in various fields of research seeking to address the demand for sequence information. However, many of these techniques rely on some kind of labeling or amplification steps. Here we investigate the intrinsic properties of tip-enhanced Raman scattering (TERS) towards the development of a novel, label-free, direct sequencing method. It is known that TERS allows the acquisition of spectral information with high lateral resolution and single-molecule sensitivity. In the presented experiments, single stranded adenine and uracil homopolymers were immobilized on different kinds of substrates (mica and gold nanoplates) and TERS experiments were conducted, which demonstrated the reproducibility of the technique. To elucidate the signal contributions from the specific nucleobases, TERS spectra were collected on single stranded calf thymus DNA with arbitrary sequence. The results show that, while the Raman signals with respect to the four nucleobases differ remarkably, specific markers can be determined for each respective base. The combination of sensitivity and reproducibility shows that the crucial demands for a sequencing procedure are met.eng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://doi.org/10.34657/4285
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/5656
dc.language.isoengeng
dc.publisherFrankfurt, M. : Beilstein-Institut zur Förderung der Chemischen Wissenschafteneng
dc.relation.doihttps://doi.org/10.3762/bjnano.2.66
dc.relation.ispartofseriesBeilstein Journal of Nanotechnology 2 (2011), 1eng
dc.relation.issn2190-4286
dc.rights.licenseCC BY 2.0 Unportedeng
dc.rights.urihttps://creativecommons.org/licenses/by/2.0/eng
dc.subjectCalf thymus DNAeng
dc.subjectDirect sequencingeng
dc.subjectDNA Sequencingeng
dc.subjectGold nanoplateseng
dc.subjectHigh-lateral resolutioneng
dc.subjectIntrinsic propertyeng
dc.subjectLabel freeeng
dc.subjectNucleobaseseng
dc.subjectRaman signaleng
dc.subjectReproducibilitieseng
dc.subjectSignal contributioneng
dc.subjectSingle-molecule sensitivityeng
dc.subjectSpectral informationeng
dc.subjectExperimentseng
dc.subjectMicaeng
dc.subjectRaman spectroscopyeng
dc.subjectGene encodingeng
dc.subject.ddc530eng
dc.titleDistinction of nucleobases - A tip-enhanced Raman approacheng
dc.typearticleeng
dc.typeTexteng
dcterms.bibliographicCitation.journalTitleBeilstein Journal of Nanotechnologyeng
tib.accessRightsopenAccesseng
wgl.contributorIPHTeng
wgl.subjectPhysikeng
wgl.typeZeitschriftenartikeleng
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