Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad

Abstract

Dyads consisting of a photochromic switch covalently linked to a fluorescent dye allow the emission from the dye to be controlled by reversible photoisomerization of the switch; one form of the switch quenches fluorescence by accepting energy from the dye. Here we investigate the use of dyads of this type for super-resolution imaging of lipid bilayers. Giant unilamellar vesicles stained with the dyads were imaged with about a two-fold resolution-enhancement compared with conventional confocal microscopy. This was achieved by exciting the fluorophore at 594 nm, using a switch activated by violet and red light (405/640 nm). This journal is © The Royal Society of Chemistry.

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Frawley, A. T., Wycisk, V., Xiong, Y., Galiani, S., Sezgin, E., Urbančič, I., et al. (2020). Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad (Cambridge : RSC). Cambridge : RSC. https://doi.org//10.1039/d0sc02447c
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CC BY 3.0 Unported