The Anticancer Efficacy of Plasma-Oxidized Saline (POS) in the Ehrlich Ascites Carcinoma Model In Vitro and In Vivo

dc.bibliographicCitation.firstPage932eng
dc.bibliographicCitation.issue8eng
dc.bibliographicCitation.journalTitleBiomedicineseng
dc.bibliographicCitation.volume9eng
dc.contributor.authorBrito, Walison Augusto Silva
dc.contributor.authorFreund, Eric
dc.contributor.authorNascimento, Thiago Daniel Henrique do
dc.contributor.authorPasqual-Melo, Gabriella
dc.contributor.authorSanches, Larissa Juliani
dc.contributor.authorDionísio, Joyce Hellen Ribeiro
dc.contributor.authorFumegali, William Capellari
dc.contributor.authorMiebach, Lea
dc.contributor.authorCecchini, Alessandra Lourenço
dc.contributor.authorBekeschus, Sander
dc.date.accessioned2022-03-18T10:59:57Z
dc.date.available2022-03-18T10:59:57Z
dc.date.issued2021
dc.description.abstractCold physical plasma, a partially ionized gas rich in reactive oxygen species (ROS), is receiving increasing interest as a novel anticancer agent via two modes. The first involves its application to cells and tissues directly, while the second uses physical plasma-derived ROS to oxidize liquids. Saline is a clinically accepted liquid, and here we explored the suitability of plasma-oxidized saline (POS) as anticancer agent technology in vitro and in vivo using the Ehrlich Ascites Carcinoma (EAC) model. EAC mainly grows as a suspension in the peritoneal cavity of mice, making this model ideally suited to test POS as a putative agent against peritoneal carcinomatosis frequently observed with colon, pancreas, and ovarium metastasis. Five POS injections led to a reduction of the tumor burden in vivo as well as in a decline of EAC cell growth and an arrest in metabolic activity ex vivo. The treatment was accompanied by a decreased antioxidant capacity of Ehrlich tumor cells and increased lipid oxidation in the ascites supernatants, while no other side effects were observed. Oxaliplatin and hydrogen peroxide were used as controls and mediated better and worse outcomes, respectively, with the former but not the latter inducing profound changes in the inflammatory milieu among 13 different cytokines investigated in ascites fluid. Modulation of inflammation in the POS group was modest but significant. These results promote POS as a promising candidate for targeting peritoneal carcinomatosis and malignant ascites and suggest EAC to be a suitable and convenient model for analyzing innovative POS approaches and combination therapies.eng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/8277
dc.identifier.urihttps://doi.org/10.34657/7315
dc.language.isoengeng
dc.publisherBasel : MDPIeng
dc.relation.doihttps://doi.org/10.3390/biomedicines9080932
dc.relation.essn2227-9059
dc.rights.licenseCC BY 4.0 Unportedeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/eng
dc.subject.ddc610eng
dc.subject.ddc570eng
dc.subject.otherEACeng
dc.subject.otherROSeng
dc.subject.othercold physical plasmaeng
dc.subject.otherkINPeneng
dc.subject.otheroncologyeng
dc.subject.otherplasma medicineeng
dc.titleThe Anticancer Efficacy of Plasma-Oxidized Saline (POS) in the Ehrlich Ascites Carcinoma Model In Vitro and In Vivoeng
dc.typeArticleeng
dc.typeTexteng
tib.accessRightsopenAccesseng
wgl.contributorINPeng
wgl.subjectMedizin, Gesundheiteng
wgl.typeZeitschriftenartikeleng
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