Functionalized carbon nanotubes as transporters for antisense oligodeoxynucleotides
dc.bibliographicCitation.firstPage | 7000 | eng |
dc.bibliographicCitation.issue | 40 | eng |
dc.bibliographicCitation.journalTitle | Journal of Materials Chemistry B | eng |
dc.bibliographicCitation.lastPage | 7008 | eng |
dc.bibliographicCitation.volume | 2 | |
dc.contributor.author | Kaufmann, Anika | |
dc.contributor.author | Kunhardt, David | |
dc.contributor.author | Cirillo, Giuseppe | |
dc.contributor.author | Hampel, Silke | |
dc.contributor.author | Schwenzer, Bernd | |
dc.date.accessioned | 2018-06-01T16:40:09Z | |
dc.date.available | 2019-06-28T07:31:16Z | |
dc.date.issued | 2014 | |
dc.description.abstract | The use of DNA-based therapeutics requires efficient delivery systems to transport the DNA to their place of action within the cell. To accomplish this, we investigated multiwalled carbon nanotubes (pristine MWCNT, p-MWCNT) functionalized with hydroxyl groups via 1,3-dipolar cycloaddition. In this way, we have obtained MWCNT-f-OH with improved stability in aqueous dispersions which is an advantageous property for their use in cellular environments. Afterwards, a carrier strand oligodeoxynucleotide (CS-ODN) was adsorbed to MWCNT-f-OH followed by hybridization with a therapeutic antisense oligodeoxynucleotide (AS-ODN). The amount of adsorbed CS-ODN, as well as the complementary AS-ODN and a non-complementary oligodeoxynucleotide (NS-ODN) as reference, was directly measured by radionuclide labeling of ODNs. We show that subsequent release of AS-ODNs and NS-ODNs was possible for MWCNT-f-OH above the melting temperature of AS-ODNs at 80 °C and under physiological conditions at different pH values at 37 °C. We also show a very low influence of p-MWCNT and MWCNT-f-OH on the cell viability of the bladder carcinoma (BCa) cell line EJ28 and that both MWCNT types were internalized by EJ28. Therefore, MWCNT-f-OH represents a promising carrier able to transport and release AS-ODNs inside cells. | eng |
dc.description.version | publishedVersion | eng |
dc.format | application/pdf | |
dc.format | application/pdf | |
dc.identifier.uri | https://doi.org/10.34657/4921 | |
dc.identifier.uri | https://oa.tib.eu/renate/handle/123456789/1429 | |
dc.language.iso | eng | eng |
dc.publisher | Cambridge : Royal Society of Chemistry | eng |
dc.relation.doi | https://doi.org/10.1039/C4TB00915K | |
dc.rights.license | CC BY-NC 3.0 Unported | eng |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/3.0/ | eng |
dc.subject.ddc | 620 | eng |
dc.subject.other | Antisense oligodeoxynucleotides | eng |
dc.subject.other | Functionalized carbon nanotubes | eng |
dc.title | Functionalized carbon nanotubes as transporters for antisense oligodeoxynucleotides | eng |
dc.type | Article | eng |
dc.type | Text | eng |
tib.accessRights | openAccess | eng |
wgl.contributor | IFWD | eng |
wgl.subject | Ingenieurwissenschaften | eng |
wgl.type | Zeitschriftenartikel | eng |