Application of new lysine-based peptide dendrimers D3K2 and D3G2 for gene delivery: Specific cytotoxicity to cancer cells and transfection in vitro

dc.bibliographicCitation.firstPage103504eng
dc.bibliographicCitation.journalTitleBioorganic chemistry : an international journaleng
dc.bibliographicCitation.volume95eng
dc.contributor.authorGorzkiewicz, Michal
dc.contributor.authorKonopka, Malgorzata
dc.contributor.authorJanaszewska, Anna
dc.contributor.authorTarasenko, Irina I.
dc.contributor.authorSheveleva, Nadezhda N.
dc.contributor.authorGajek, Arkadiusz
dc.contributor.authorNeelov, Igor M.
dc.contributor.authorKlajnert-Maculewicz, Barbara
dc.date.accessioned2021-09-14T09:17:43Z
dc.date.available2021-09-14T09:17:43Z
dc.date.issued2020
dc.description.abstractIn order to enhance intracellular uptake and accumulation of therapeutic nucleic acids for improved gene therapy methods, numerous delivery vectors have been elaborated. Based on their origin, gene carriers are generally classified as viral or non-viral vectors. Due to their significantly reduced immunogenicity and highly optimized methods of synthesis, nanoparticles (especially those imitating natural biomolecules) constitute a promising alternative for virus-based delivery devices. Thus, we set out to develop innovative peptide dendrimers for clinical application as transfection agents and gene carriers. In the present work we describe the synthesis of two novel lysine-based dendritic macromolecules (D3K2 and D3G2) and their initial characterization for cytotoxicity/genotoxicity and transfection potential in two human cell line models: cervix adenocarcinoma (HeLa) and microvascular endothelial (HMEC-1). This approach allowed us to identify more cationic D3K2 as potent delivery agent, being able to increase intracellular accumulation of large nucleic acid molecules such as plasmids. Moreover, the dendrimers exhibited specific cytotoxicity towards cancer cell line without showing significant toxic effects on normal cells. These observations are promising prognosis for future clinical application of this type of nanoparticles. © 2019 The Authorseng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/6794
dc.identifier.urihttps://doi.org/10.34657/5841
dc.language.isoengeng
dc.publisherSan Diego, Calif. : Elseviereng
dc.relation.doihttps://doi.org/10.1016/j.bioorg.2019.103504
dc.relation.essn1090-2120
dc.relation.issn0045-2068
dc.rights.licenseCC BY-NC-ND 4.0 Unportedeng
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/eng
dc.subject.ddc540eng
dc.subject.otherd3g2 peptideeng
dc.subject.otherd3k2 peptideeng
dc.subject.otherdendrimereng
dc.subject.otherlipofectamineeng
dc.subject.othernanocarriereng
dc.subject.otherplasmid DNAeng
dc.subject.otherpolylysineeng
dc.subject.othersynthetic peptideeng
dc.subject.otherunclassified drugeng
dc.subject.otherapoptosiseng
dc.subject.otherArticleeng
dc.subject.othercancer celleng
dc.subject.othercomet assayeng
dc.subject.othercontrolled studyeng
dc.subject.othercytotoxicityeng
dc.subject.otherDNA damageeng
dc.subject.otherfemaleeng
dc.subject.othergenetic transfectioneng
dc.subject.othergenotoxicityeng
dc.subject.otherHeLa cell lineeng
dc.subject.otherHMEC-1 cell lineeng
dc.subject.otherhumaneng
dc.subject.otherhuman celleng
dc.subject.otherin vitro gene transfereng
dc.subject.otherin vitro studyeng
dc.subject.othermacromoleculeeng
dc.subject.otherMTT assayeng
dc.subject.othernonviral gene delivery systemeng
dc.subject.otherplasmideng
dc.subject.otherpriority journaleng
dc.subject.otherTUNEL assayeng
dc.titleApplication of new lysine-based peptide dendrimers D3K2 and D3G2 for gene delivery: Specific cytotoxicity to cancer cells and transfection in vitroeng
dc.typeArticleeng
dc.typeTexteng
tib.accessRightsopenAccesseng
wgl.contributorIPFeng
wgl.subjectChemieeng
wgl.typeZeitschriftenartikeleng
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