Gas Plasma Technology Augments Ovalbumin Immunogenicity and OT-II T Cell Activation Conferring Tumor Protection in Mice
dc.bibliographicCitation.firstPage | 2003395 | |
dc.bibliographicCitation.issue | 10 | |
dc.bibliographicCitation.journalTitle | Advanced Science | eng |
dc.bibliographicCitation.volume | 8 | |
dc.contributor.author | Clemen, Ramona | |
dc.contributor.author | Freund, Eric | |
dc.contributor.author | Mrochen, Daniel | |
dc.contributor.author | Miebach, Lea | |
dc.contributor.author | Schmidt, Anke | |
dc.contributor.author | Rauch, Bernhard H. | |
dc.contributor.author | Lackmann, Jan‐Wilm | |
dc.contributor.author | Martens, Ulrike | |
dc.contributor.author | Wende, Kristian | |
dc.contributor.author | Lalk, Michael | |
dc.contributor.author | Delcea, Mihaela | |
dc.contributor.author | Bröker, Barbara M. | |
dc.contributor.author | Bekeschus, Sander | |
dc.date.accessioned | 2023-05-25T10:24:56Z | |
dc.date.available | 2023-05-25T10:24:56Z | |
dc.date.issued | 2021 | |
dc.description.abstract | Reactive oxygen species (ROS/RNS) are produced during inflammation and elicit protein modifications, but the immunological consequences are largely unknown. Gas plasma technology capable of generating an unmatched variety of ROS/RNS is deployed to mimic inflammation and study the significance of ROS/RNS modifications using the model protein chicken ovalbumin (Ova vs oxOva). Dynamic light scattering and circular dichroism spectroscopy reveal structural modifications in oxOva compared to Ova. T cells from Ova-specific OT-II but not from C57BL/6 or SKH-1 wild type mice presents enhanced activation after Ova addition. OxOva exacerbates this activation when administered ex vivo or in vivo, along with an increased interferon-gamma production, a known anti-melanoma agent. OxOva vaccination of wild type mice followed by inoculation of syngeneic B16F10 Ova-expressing melanoma cells shows enhanced T cell number and activation, decreased tumor burden, and elevated numbers of antigen-presenting cells when compared to their Ova-vaccinated counterparts. Analysis of oxOva using mass spectrometry identifies three hot spots regions rich in oxidative modifications that are associated with the increased T cell activation. Using Ova as a model protein, the findings suggest an immunomodulating role of multi-ROS/RNS modifications that may spur novel research lines in inflammation research and for vaccination strategies in oncology. | eng |
dc.description.version | publishedVersion | eng |
dc.identifier.uri | https://oa.tib.eu/renate/handle/123456789/12179 | |
dc.identifier.uri | http://dx.doi.org/10.34657/11211 | |
dc.language.iso | eng | |
dc.publisher | Weinheim : Wiley-VCH | |
dc.relation.doi | https://doi.org/10.1002/advs.202003395 | |
dc.relation.essn | 2198-3844 | |
dc.relation.issn | 2198-3844 | |
dc.rights.license | CC BY 4.0 Unported | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.subject.ddc | 500 | |
dc.subject.ddc | 600 | |
dc.subject.ddc | 624 | |
dc.subject.other | kINPen | eng |
dc.subject.other | ovalbumin | eng |
dc.subject.other | oxPTM | eng |
dc.subject.other | ROS | eng |
dc.subject.other | vaccines | eng |
dc.title | Gas Plasma Technology Augments Ovalbumin Immunogenicity and OT-II T Cell Activation Conferring Tumor Protection in Mice | eng |
dc.type | Article | eng |
dc.type | Text | eng |
tib.accessRights | openAccess | |
wgl.contributor | INP | |
wgl.subject | Medizin, Gesundheit | ger |
wgl.type | Zeitschriftenartikel | ger |
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