Nanoscale dynamics of cholesterol in the cell membrane

dc.bibliographicCitation.firstPage12599eng
dc.bibliographicCitation.issue34eng
dc.bibliographicCitation.lastPage12609eng
dc.bibliographicCitation.volume294eng
dc.contributor.authorPinkwart, Kerstin
dc.contributor.authorSchneider, Falk
dc.contributor.authorLukoseviciute, Martyna
dc.contributor.authorSauka-Spengler, Tatjana
dc.contributor.authorLyman, Edward
dc.contributor.authorEggeling, Christian
dc.contributor.authorSezgin, Erdinc
dc.date.accessioned2021-11-09T12:11:35Z
dc.date.available2021-11-09T12:11:35Z
dc.date.issued2019
dc.description.abstractCholesterol constitutes ~30-40% of the mammalian plasma membrane, a larger fraction than of any other single component. It is a major player in numerous signaling processes as well as in shaping molecular membrane architecture. However, our knowledge of the dynamics of cholesterol in the plasma membrane is limited, restricting our understanding of the mechanisms regulating its involvement in cell signaling. Here, we applied advanced fluorescence imaging and spectroscopy approaches on in vitro (model membranes) and in vivo (live cells and embryos) membranes as well as in silico analysis to systematically study the nanoscale dynamics of cholesterol in biological membranes. Our results indicate that cholesterol diffuses faster than phospholipids in live membranes, but not in model membranes. Interestingly, a detailed statistical diffusion analysis suggested two-component diffusion for cholesterol in the plasma membrane of live cells. One of these components was similar to a freely diffusing phospholipid analogue, whereas the other one was significantly faster. When a cholesterol analogue was localized to the outer leaflet only, the fast diffusion of cholesterol disappeared, and it diffused similarly to phospholipids. Overall, our results suggest that cholesterol diffusion in the cell membrane is heterogeneous and that this diffusional heterogeneity is due to cholesterol's nanoscale interactions and localization in the membrane. © 2019 Pinkwart et al. Published by The American Society for Biochemistry and Molecular Biology, Inc.eng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/7221
dc.identifier.urihttps://doi.org/10.34657/6268
dc.language.isoengeng
dc.publisherBethesda, Md. : ASBMB Publicationseng
dc.relation.doihttps://doi.org/10.1074/jbc.RA119.009683
dc.relation.essn1083-351X
dc.relation.ispartofseriesThe journal of biological chemistry : JBC 294 (2019), Nr. 34eng
dc.rights.licenseCC BY 4.0 Unportedeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/eng
dc.subjectCholesteroleng
dc.subjectplasma membraneeng
dc.subjectcell signalingeng
dc.subject.ddc570eng
dc.subject.ddc540eng
dc.titleNanoscale dynamics of cholesterol in the cell membraneeng
dc.typearticleeng
dc.typeTexteng
dcterms.bibliographicCitation.journalTitleThe journal of biological chemistry : JBCeng
tib.accessRightsopenAccesseng
wgl.contributorIPHTeng
wgl.subjectChemieeng
wgl.typeZeitschriftenartikeleng
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