Silk Nanoparticle Manufacture in Semi-Batch Format
dc.bibliographicCitation.firstPage | 6748 | eng |
dc.bibliographicCitation.issue | 12 | eng |
dc.bibliographicCitation.journalTitle | ACS Biomaterials Science and Engineering | eng |
dc.bibliographicCitation.lastPage | 6759 | eng |
dc.bibliographicCitation.volume | 6 | eng |
dc.contributor.author | Matthew, Saphia A.L. | |
dc.contributor.author | Totten, John D. | |
dc.contributor.author | Phuagkhaopong, Suttinee | |
dc.contributor.author | Egan, Gemma | |
dc.contributor.author | Witte, Kimia | |
dc.contributor.author | Perrie, Yvonne | |
dc.contributor.author | Seib, F. Philipp | |
dc.date.accessioned | 2021-09-02T07:01:37Z | |
dc.date.available | 2021-09-02T07:01:37Z | |
dc.date.issued | 2020 | |
dc.description.abstract | Silk nanoparticles have demonstrated utility across a range of biomedical applications, especially as drug delivery vehicles. Their fabrication by bottom-up methods such as nanoprecipitation, rather than top-down manufacture, can improve critical nanoparticle quality attributes. Here, we establish a simple semi-batch method using drop-by-drop nanoprecipitation at the lab scale that reduces special-cause variation and improves mixing efficiency. The stirring rate was an important parameter affecting nanoparticle size and yield (400 < 200 < 0 rpm), while the initial dropping height (5.5 vs 7.5 cm) directly affected nanoparticle yield. Varying the nanoparticle standing time in the mother liquor between 0 and 24 h did not significantly affect nanoparticle physicochemical properties, indicating that steric and charge stabilizations result in high-energy barriers for nanoparticle growth. Manufacture across all tested formulations achieved nanoparticles between 104 and 134 nm in size with high β-sheet content, spherical morphology, and stability in aqueous media for over 1 month at 4 °C. This semi-automated drop-by-drop, semi-batch silk desolvation offers an accessible, higher-throughput platform for standardization of parameters that are difficult to control using manual methodologies. © 2020 American Chemical Society. | eng |
dc.description.version | publishedVersion | eng |
dc.identifier.uri | https://oa.tib.eu/renate/handle/123456789/6659 | |
dc.identifier.uri | https://doi.org/10.34657/5706 | |
dc.language.iso | eng | eng |
dc.publisher | Washington, DC : ACS Publications | eng |
dc.relation.doi | https://doi.org/10.1021/acsbiomaterials.0c01028 | |
dc.relation.essn | 2373-9878 | |
dc.rights.license | CC BY 4.0 Unported | eng |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | eng |
dc.subject.ddc | 540 | eng |
dc.subject.other | biopolymer | eng |
dc.subject.other | desolvation | eng |
dc.subject.other | nanoparticle | eng |
dc.subject.other | nanoprecipitation | eng |
dc.title | Silk Nanoparticle Manufacture in Semi-Batch Format | eng |
dc.type | Article | eng |
dc.type | Text | eng |
tib.accessRights | openAccess | eng |
wgl.contributor | IPF | eng |
wgl.subject | Chemie | eng |
wgl.type | Zeitschriftenartikel | eng |
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