Singlet-Oxygen Generation by Peroxidases and Peroxygenases for Chemoenzymatic Synthesis

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dc.bibliographicCitation.firstPage398eng
dc.bibliographicCitation.issue2eng
dc.bibliographicCitation.journalTitleChemBioChem : a European journal of chemical biologyeng
dc.bibliographicCitation.lastPage407eng
dc.bibliographicCitation.volume22eng
dc.contributor.authorIngenbosch, Kim N.
dc.contributor.authorQuint, Stephan
dc.contributor.authorDyllick-Brenzinger, Melanie
dc.contributor.authorWunschik, Dennis S.
dc.contributor.authorKiebist, Jan
dc.contributor.authorSüss, Philipp
dc.contributor.authorLiebelt, Ute
dc.contributor.authorZuhse, Ralf
dc.contributor.authorMenyes, Ulf
dc.contributor.authorScheibner, Katrin
dc.contributor.authorMayer, Christian
dc.contributor.authorOpwis, Klaus
dc.contributor.authorGutmann, Jochen S.
dc.contributor.authorHoffmann-Jacobsen, Kerstin
dc.date.accessioned2021-09-10T06:43:09Z
dc.date.available2021-09-10T06:43:09Z
dc.date.issued2020
dc.description.abstractSinglet oxygen is a reactive oxygen species undesired in living cells but a rare and valuable reagent in chemical synthesis. We present a fluorescence spectroscopic analysis of the singlet-oxygen formation activity of commercial peroxidases and novel peroxygenases. Singlet-oxygen sensor green (SOSG) is used as fluorogenic singlet oxygen trap. Establishing a kinetic model for the reaction cascade to the fluorescent SOSG endoperoxide permits a kinetic analysis of enzymatic singlet-oxygen formation. All peroxidases and peroxygenases show singlet-oxygen formation. No singlet oxygen activity could be found for any catalase under investigation. Substrate inhibition is observed for all reactive enzymes. The commercial dye-decolorizing peroxidase industrially used for dairy bleaching shows the highest singlet-oxygen activity and the lowest inhibition. This enzyme was immobilized on a textile carrier and successfully applied for a chemical synthesis. Here, ascaridole was synthesized via enzymatically produced singlet oxygen. © 2020 Wiley-VCH GmbHeng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/6779
dc.identifier.urihttps://doi.org/10.34657/5826
dc.language.isoengeng
dc.publisherWeinheim : Wiley-VCHeng
dc.relation.doihttps://doi.org/10.1002/cbic.202000326
dc.relation.essn1439-7633
dc.relation.issn1439-4227
dc.rights.licenseCC BY 4.0 Unportedeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/eng
dc.subject.ddc540eng
dc.subject.otherchemo-enzymatic synthesiseng
dc.subject.otherfluorescenceeng
dc.subject.otherperoxidaseeng
dc.subject.otherperoxygenaseeng
dc.subject.othersinglet oxygeneng
dc.subject.othersinglet oxygen sensor greeneng
dc.titleSinglet-Oxygen Generation by Peroxidases and Peroxygenases for Chemoenzymatic Synthesiseng
dc.typeArticleeng
dc.typeTexteng
tib.accessRightsopenAccesseng
wgl.contributorINPeng
wgl.subjectChemieeng
wgl.typeZeitschriftenartikeleng
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