On the impact of competing intra- and intermolecular triplet-state quenching on photobleaching and photoswitching kinetics of organic fluorophores
dc.bibliographicCitation.firstPage | 3721 | eng |
dc.bibliographicCitation.issue | 7 | eng |
dc.bibliographicCitation.journalTitle | Physical chemistry, chemical physics : PCCP | eng |
dc.bibliographicCitation.lastPage | 3733 | eng |
dc.bibliographicCitation.volume | 21 | eng |
dc.contributor.author | Smit, Jochem H. | |
dc.contributor.author | van der Velde, Jasper H. M. | |
dc.contributor.author | Huang, Jingyi | |
dc.contributor.author | Trauschke, Vanessa | |
dc.contributor.author | Henrikus, Sarah S. | |
dc.contributor.author | Chen, Si | |
dc.contributor.author | Eleftheriadis, Nikolaos | |
dc.contributor.author | Warszawik, Eliza M. | |
dc.contributor.author | Herrmann, Andreas | |
dc.contributor.author | Cordes, Thorben | |
dc.date.accessioned | 2022-04-05T12:06:18Z | |
dc.date.available | 2022-04-05T12:06:18Z | |
dc.date.issued | 2019 | |
dc.description.abstract | While buffer cocktails remain the most commonly used method for photostabilization and photoswitching of fluorescent markers, intramolecular triplet-state quenchers emerge as an alternative strategy to impart fluorophores with ‘self-healing’ or even functional properties such as photoswitching. In this contribution, we evaluated combinations of both approaches and show that inter- and intramolecular triplet-state quenching processes compete with each other. We find that although the rate of triplet-state quenching is additive, the photostability is limited by the faster pathway. Often intramolecular processes dominate the photophysical situation for combinations of covalently-linked and solution-based photostabilizers and photoswitching agents. Furthermore we show that intramolecular photostabilizers can protect fluorophores from reversible off-switching events caused by solution-additives, which was previously misinterpreted as photobleaching. Our studies also provide practical guidance for usage of photostabilizer–dye conjugates for STORM-type super-resolution microscopy permitting the exploitation of their improved photophysics for increased spatio-temporal resolution. Finally, we provide evidence that the biochemical environment, e.g., proximity of aromatic amino-acids such as tryptophan, reduces the photostabilization efficiency of commonly used buffer cocktails. Not only have our results important implications for a deeper mechanistic understanding of self-healing dyes, but they will provide a general framework to select label positions for optimal and reproducible photostability or photoswitching kinetics in different biochemical environments. | eng |
dc.description.version | publishedVersion | eng |
dc.identifier.uri | https://oa.tib.eu/renate/handle/123456789/8586 | |
dc.identifier.uri | https://doi.org/10.34657/7624 | |
dc.language.iso | eng | eng |
dc.publisher | Cambridge : RSC Publ. | eng |
dc.relation.doi | https://doi.org/10.1039/c8cp05063e | |
dc.relation.essn | 1463-9084 | |
dc.rights.license | CC BY-NC 3.0 Unported | eng |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/3.0/ | eng |
dc.subject.ddc | 540 | eng |
dc.subject.other | Additives | eng |
dc.subject.other | Amino acids | eng |
dc.subject.other | Fluorophores | eng |
dc.subject.other | Photobleaching | eng |
dc.subject.other | Photophysics | eng |
dc.subject.other | Self-healing materials | eng |
dc.subject.other | Aromatic amino acid | eng |
dc.subject.other | Fluorescent markers | eng |
dc.subject.other | Functional properties | eng |
dc.subject.other | Intramolecular process | eng |
dc.subject.other | Intramolecular triplets | eng |
dc.subject.other | Organic fluorophores | eng |
dc.subject.other | Spatio-temporal resolution | eng |
dc.subject.other | Super-resolution microscopy | eng |
dc.subject.other | Quenching | eng |
dc.title | On the impact of competing intra- and intermolecular triplet-state quenching on photobleaching and photoswitching kinetics of organic fluorophores | eng |
dc.type | Article | eng |
dc.type | Text | eng |
tib.accessRights | openAccess | eng |
wgl.contributor | DWI | eng |
wgl.subject | Chemie | eng |
wgl.type | Zeitschriftenartikel | eng |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- On_the_impact_of_competing.pdf
- Size:
- 4.5 MB
- Format:
- Adobe Portable Document Format
- Description: