Murine Macrophages Modulate Their Inflammatory Profile in Response to Gas Plasma-Inactivated Pancreatic Cancer Cells
dc.bibliographicCitation.firstPage | 2525 | eng |
dc.bibliographicCitation.issue | 11 | eng |
dc.bibliographicCitation.journalTitle | Cancers | eng |
dc.bibliographicCitation.volume | 13 | eng |
dc.contributor.author | Khabipov, Aydar | |
dc.contributor.author | Freund, Eric | |
dc.contributor.author | Liedtke, Kim Rouven | |
dc.contributor.author | Käding, Andre | |
dc.contributor.author | Riese, Janik | |
dc.contributor.author | van der Linde, Julia | |
dc.contributor.author | Kersting, Stephan | |
dc.contributor.author | Partecke, Lars-Ivo | |
dc.contributor.author | Bekeschus, Sander | |
dc.date.accessioned | 2022-03-23T14:24:43Z | |
dc.date.available | 2022-03-23T14:24:43Z | |
dc.date.issued | 2021 | |
dc.description.abstract | Macrophages and immuno-modulation play a dominant role in the pathology of pancreatic cancer. Gas plasma is a technology recently suggested to demonstrate anticancer efficacy. To this end, two murine cell lines were employed to analyze the inflammatory consequences of plasma-treated pancreatic cancer cells (PDA) on macrophages using the kINPen plasma jet. Plasma treatment decreased the metabolic activity, viability, and migratory activity in an ROS- and treatment time-dependent manner in PDA cells in vitro. These results were confirmed in pancreatic tumors grown on chicken embryos in the TUM-CAM model (in ovo). PDA cells promote tumor-supporting M2 macrophage polarization and cluster formation. Plasma treatment of PDA cells abrogated this cluster formation with a mixed M1/M2 phenotype observed in such co-cultured macrophages. Multiplex chemokine and cytokine quantification showed a marked decrease of the neutrophil chemoattractant CXCL1, IL6, and the tumor growth supporting TGFβ and VEGF in plasma-treated compared to untreated co-culture settings. At the same time, macrophage-attractant CCL4 and MCP1 release were profoundly enhanced. These cellular and secretome data suggest that the plasma-inactivated PDA6606 cells modulate the inflammatory profile of murine RAW 264.7 macrophages favorably, which may support plasma cancer therapy. | eng |
dc.description.version | publishedVersion | eng |
dc.identifier.uri | https://oa.tib.eu/renate/handle/123456789/8337 | |
dc.identifier.uri | https://doi.org/10.34657/7375 | |
dc.language.iso | eng | eng |
dc.publisher | Basel : MDPI | eng |
dc.relation.doi | https://doi.org/10.3390/cancers13112525 | |
dc.relation.essn | 2072-6694 | |
dc.rights.license | CC BY 4.0 Unported | eng |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | eng |
dc.subject.ddc | 610 | eng |
dc.subject.other | CAP | eng |
dc.subject.other | PDA6606 | eng |
dc.subject.other | cold atmospheric pressure plasma | eng |
dc.subject.other | cold physical plasma | eng |
dc.subject.other | kINPen | eng |
dc.subject.other | metastasis | eng |
dc.subject.other | plasma medicine | eng |
dc.subject.other | tumor microenvironment (TME) | eng |
dc.title | Murine Macrophages Modulate Their Inflammatory Profile in Response to Gas Plasma-Inactivated Pancreatic Cancer Cells | eng |
dc.type | Article | eng |
dc.type | Text | eng |
tib.accessRights | openAccess | eng |
wgl.contributor | INP | eng |
wgl.subject | Medizin, Gesundheit | eng |
wgl.type | Zeitschriftenartikel | eng |
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