Bacterial community and PHB-accumulating bacteria associated with the wall and specialized niches of the hindgut of the forest cockchafer (Melolontha hippocastani)

dc.bibliographicCitation.firstPage291eng
dc.bibliographicCitation.volume8eng
dc.contributor.authorAlonso-Pernas, Pol
dc.contributor.authorArias-Cordero, Erika
dc.contributor.authorNovoselov, Alexey
dc.contributor.authorGroße, Christina
dc.contributor.authorRybak, Jürgen
dc.contributor.authorKaltenpoth, Martin
dc.contributor.authorWestermann, Martin
dc.contributor.authorNeugebauer, Ute
dc.contributor.authorBoland, Wilhelm
dc.date.accessioned2019-12-19T10:53:40Z
dc.date.available2019-12-19T10:53:40Z
dc.date.issued2017
dc.description.abstractA characterization of the bacterial community of the hindgut wall of two larval and the adult stages of the forest cockchafer (Melolontha hippocastani) was carried out using amplicon sequencing of the 16S rRNA gene fragment. We found that, in second-instar larvae, Caulobacteraceae and Pseudomonadaceae showed the highest relative abundances, while in third-instar larvae, the dominant families were Porphyromonadaceae and Bacteroidales-related. In adults, an increase of the relative abundance of Bacteroidetes, Proteobacteria (γ- and δ- classes) and the family Enterococcaceae (Firmicutes) was observed. This suggests that the composition of the hindgut wall community may depend on the insect’s life stage. Additionally, specialized bacterial niches hitherto very poorly described in the literature were spotted at both sides of the distal part of the hindgut chamber. We named these structures “pockets.” Amplicon sequencing of the 16S rRNA gene fragment revealed that the pockets contained a different bacterial community than the surrounding hindgut wall, dominated by Alcaligenaceae and Micrococcaceae-related families. Poly-β-hydroxybutyrate (PHB) accumulation in the pocket was suggested in isolated Achromobacter sp. by Nile Blue staining, and confirmed by gas chromatography–mass spectrometry analysis (GC-MS) on cultured bacterial mass and whole pocket tissue. Raman micro-spectroscopy allowed to visualize the spatial distribution of PHB accumulating bacteria within the pocket tissue. The presence of this polymer might play a role in the colonization of these specialized niches.eng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://doi.org/10.34657/5128
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/4712
dc.language.isoengeng
dc.publisherLausanne : Frontiers Mediaeng
dc.relation.doihttps://doi.org/10.3389/fmicb.2017.00291
dc.relation.ispartofseriesFrontiers in Microbiology 8 (2017)eng
dc.rights.licenseCC BY 4.0 Unportedeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/eng
dc.subjecthindguteng
dc.subjectMelolontha hippocastanieng
dc.subjectgut bacteriaeng
dc.subjectpoly-b-hydroxybutyrateeng
dc.subjectPHBeng
dc.subjectAchromobactereng
dc.subjectRaman microscopyeng
dc.subject.ddc570eng
dc.titleBacterial community and PHB-accumulating bacteria associated with the wall and specialized niches of the hindgut of the forest cockchafer (Melolontha hippocastani)eng
dc.typearticleeng
dc.typeTexteng
dcterms.bibliographicCitation.journalTitleFrontiers in Microbiologyeng
tib.accessRightsopenAccesseng
wgl.contributorIPHTeng
wgl.subjectIngenieurwissenschafteneng
wgl.typeZeitschriftenartikeleng
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