Devitalization of Glioblastoma Cancer Cells by Non-invasive Physical Plasma: Modulation of Proliferative Signalling Cascades

dc.bibliographicCitation.date2023
dc.bibliographicCitation.firstPage7
dc.bibliographicCitation.issue1
dc.bibliographicCitation.lastPage18
dc.bibliographicCitation.volume43
dc.contributor.authorLehmann, Sebastian
dc.contributor.authorBien-Möller, Sandra
dc.contributor.authorMarx, Sascha
dc.contributor.authorBekeschus, Sander
dc.contributor.authorSchroeder, Henry W.S.
dc.contributor.authorMustea, Alexander
dc.contributor.authorStope, Matthias B.
dc.date.accessioned2023-02-21T06:32:55Z
dc.date.available2023-02-21T06:32:55Z
dc.date.issued2022
dc.description.abstractBackground/Aim: Glioblastoma (GBM) is the most common and most lethal type of cancer of the central nervous system in adults. Despite aggressive treatment, which is based on surgical resection, if possible, followed by radiation and chemotherapy, a high recurrence rate and therapy resistance is observed. Thus, additional innovative therapies are urgently needed to improve the poor median survival of only 15 months. Treatment of solid tumours with non-invasive physical plasma (NIPP) represents such a novel and innovative anticancer procedure. Materials and Methods: In this study, we investigated the effect of NIPP, an ionized argon gas, on the in vitro growth of human GBM cell lines, LN-18 and U-87 MG. Proliferation was measured by live cell count. Subsequently, proliferative factors were analysed at the level of nucleic acids (polymerase chain reaction) and proteins (western blotting). Results: For both GBM lines, a treatment time-dependent decrease in growth was observed compared to controls. Additionally, NIPP treatment resulted in reduced rates of AKT serine/threonine kinase 1 (AKT1) and extracellular-regulated kinase 1/2 ERK1/2 expression, whereas expression of p21, proliferating cell nuclear antigen, and heat-shock proteins 90α and 90β was not affected. In both cell lines, a strong increase in expression of tumour-suppressive microRNA-1 (miR-1) was detected after exposure to NIPP. Conclusion: Our results demonstrated that NIPP is able to efficiently attenuate growth of GBM cells and suggest AKT1, ERK1/2 and miR-1 to be pivotal factors of NIPP-modulated cellular signalling. Translated into the clinical setting, NIPP may represent a promising option for the treatment of GBM.eng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/11463
dc.identifier.urihttp://dx.doi.org/10.34657/10497
dc.language.isoeng
dc.publisherAttiki : [Verlag nicht ermittelbar]
dc.relation.doihttps://doi.org/10.21873/anticanres.16128
dc.relation.essn1791-7530
dc.relation.ispartofseriesAnticancer research : international journal of cancer research and treatment 43 (2023), Nr. 1
dc.relation.issn0250-7005
dc.rights.licenseCC BY-NC-ND 4.0 Unported
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAKT1eng
dc.subjectcold atmosphere pressure plasmaeng
dc.subjectCold atmospheric plasmaeng
dc.subjectmicroRNA-1eng
dc.subjecttissue tolerable plasmaeng
dc.subject.ddc610
dc.titleDevitalization of Glioblastoma Cancer Cells by Non-invasive Physical Plasma: Modulation of Proliferative Signalling Cascadeseng
dc.typearticle
dc.typeText
dcterms.bibliographicCitation.journalTitleAnticancer research : international journal of cancer research and treatment
tib.accessRightsopenAccess
wgl.contributorINP
wgl.subjectMedizin, Gesundheitger
wgl.typeZeitschriftenartikelger
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