Differential influence of components resulting from atmospheric-pressure plasma on integrin expression of human HaCaT keratinocytes

Adequate chronic wound healing is a major problem in medicine. A new solution might be non-thermal atmospheric-pressure plasma effectively inactivating microorganisms and influencing cells in wound healing. Plasma components as, for example, radicals can affect cells differently. HaCaT keratinocytes were treated with Dielectric Barrier Discharge plasma (DBD/air, DBD/argon), ozone or hydrogen peroxide to find the components responsible for changes in integrin expression, intracellular ROS formation or apoptosis induction. Dependent on plasma treatment time reduction of recovered cells was observed with no increase of apoptotic cells, but breakdown of mitochondrial membrane potential. DBD/air plasma increased integrins and intracellular ROS. DBD/argon caused minor changes. About 100 ppm ozone did not influence integrins. Hydrogen peroxide caused similar effects compared to DBD/air plasma. In conclusion, effects depended on working gas and exposure time to plasma. Short treatment cycles did neither change integrins nor induce apoptosis or ROS. Longer treatments changed integrins as important for influencing wound healing. Plasma effects on integrins are rather attributed to induction of other ROS than to generation of ozone. Changes of integrins by plasma may provide new solutions of improving wound healing, however, conditions are needed which allow initiating the relevant influence on integrins without being cytotoxic to cells.

Keywords

argon, hydrogen peroxide, hydroxyl radical, integrin, ozone, reactive oxygen metabolite, integrin, plasma gas, apoptosis, article, atmospheric pressure plasma, cell adhesion, cell migration, cell proliferation, controlled study, human, human cell, keratinocyte, mitochondrial membrane potential, protein expression, wound care, wound healing, cell line, chemistry, gene expression regulation, keratinocyte, metabolism, plasma gas, radiation response, mitochondrial membrane potential, radiation exposure, Apoptosis, Cell Line, Gene Expression Regulation, Humans, Hydrogen Peroxide, Integrins, Keratinocytes, Membrane Potential, Mitochondrial, Ozone, Plasma Gases, Reactive Oxygen Species, Wound Healing, Apoptosis, Cell Line, Gene Expression Regulation, Humans, Hydrogen Peroxide, Integrins, Keratinocytes, Membrane Potential, Mitochondrial, Ozone, Plasma Gases, Reactive Oxygen Species, Wound Healing

URI

https://doi.org/10.34657/4278
https://oa.tib.eu/renate/handle/123456789/5649

Citation

Haertel, B., Straßenburg, S., Oehmigen, K., Wende, K., Von Woedtke, T., & Lindequist, U. (2013). Differential influence of components resulting from atmospheric-pressure plasma on integrin expression of human HaCaT keratinocytes. 2013. https://doi.org//10.1155/2013/761451

Collections

Medizin

License

CC BY 3.0 Unported

https://creativecommons.org/licenses/by/3.0/

Full item page