Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction

dc.bibliographicCitation.firstPage4315eng
dc.bibliographicCitation.journalTitleNature Communicationseng
dc.bibliographicCitation.lastPage969eng
dc.bibliographicCitation.volume10eng
dc.contributor.authorMarkwirth, A
dc.contributor.authorLachetta, Mario
dc.contributor.authorMönkemöller, V.
dc.contributor.authorHeintzmann, Rainer
dc.contributor.authorHübner, Wolfgang
dc.contributor.authorHuser, Thomas
dc.contributor.authorMüller, Marcel
dc.date.accessioned2020-01-03T12:17:59Z
dc.date.available2020-01-03T12:17:59Z
dc.date.issued2019
dc.description.abstractSuper-resolved structured illumination microscopy (SR-SIM) is among the fastest fluorescence microscopy techniques capable of surpassing the optical diffraction limit. Current custom-build instruments are able to deliver two-fold resolution enhancement with high acquisition speed. SR-SIM is usually a two-step process, with raw-data acquisition and subsequent, time-consuming post-processing for image reconstruction. In contrast, wide-field and (multi-spot) confocal techniques produce high-resolution images instantly. Such immediacy is also possible with SR-SIM, by tight integration of a video-rate capable SIM with fast reconstruction software. Here we present instant SR-SIM by VIGOR (Video-rate Immediate GPU-accelerated Open-Source Reconstruction). We demonstrate multi-color SR-SIM at video frame-rates, with less than 250 ms delay between measurement and reconstructed image display. This is achieved by modifying and extending high-speed SR-SIM image acquisition with a new, GPU-enhanced, network-enabled image-reconstruction software. We demonstrate high-speed surveying of biological samples in multiple colors and live imaging of moving mitochondria as an example of intracellular dynamics.eng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://doi.org/10.34657/65
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/4794
dc.language.isoengeng
dc.publisherBerlin : Nature Publishingeng
dc.relation.doihttps://doi.org/10.1038/s41467-019-12165-x
dc.rights.licenseCC BY 4.0 Unportedeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/eng
dc.subject.ddc620eng
dc.subject.otherSR-SIMeng
dc.subject.othermicroscopyeng
dc.subject.otherVIGOReng
dc.titleVideo-rate multi-color structured illumination microscopy with simultaneous real-time reconstructioneng
dc.typeArticleeng
dc.typeTexteng
tib.accessRightsopenAccesseng
wgl.contributorIPHTeng
wgl.subjectIngenieurwissenschafteneng
wgl.typeZeitschriftenartikeleng
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