Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction
dc.bibliographicCitation.firstPage | 4315 | eng |
dc.bibliographicCitation.journalTitle | Nature Communications | eng |
dc.bibliographicCitation.lastPage | 969 | eng |
dc.bibliographicCitation.volume | 10 | eng |
dc.contributor.author | Markwirth, A | |
dc.contributor.author | Lachetta, Mario | |
dc.contributor.author | Mönkemöller, V. | |
dc.contributor.author | Heintzmann, Rainer | |
dc.contributor.author | Hübner, Wolfgang | |
dc.contributor.author | Huser, Thomas | |
dc.contributor.author | Müller, Marcel | |
dc.date.accessioned | 2020-01-03T12:17:59Z | |
dc.date.available | 2020-01-03T12:17:59Z | |
dc.date.issued | 2019 | |
dc.description.abstract | Super-resolved structured illumination microscopy (SR-SIM) is among the fastest fluorescence microscopy techniques capable of surpassing the optical diffraction limit. Current custom-build instruments are able to deliver two-fold resolution enhancement with high acquisition speed. SR-SIM is usually a two-step process, with raw-data acquisition and subsequent, time-consuming post-processing for image reconstruction. In contrast, wide-field and (multi-spot) confocal techniques produce high-resolution images instantly. Such immediacy is also possible with SR-SIM, by tight integration of a video-rate capable SIM with fast reconstruction software. Here we present instant SR-SIM by VIGOR (Video-rate Immediate GPU-accelerated Open-Source Reconstruction). We demonstrate multi-color SR-SIM at video frame-rates, with less than 250 ms delay between measurement and reconstructed image display. This is achieved by modifying and extending high-speed SR-SIM image acquisition with a new, GPU-enhanced, network-enabled image-reconstruction software. We demonstrate high-speed surveying of biological samples in multiple colors and live imaging of moving mitochondria as an example of intracellular dynamics. | eng |
dc.description.version | publishedVersion | eng |
dc.identifier.uri | https://doi.org/10.34657/65 | |
dc.identifier.uri | https://oa.tib.eu/renate/handle/123456789/4794 | |
dc.language.iso | eng | eng |
dc.publisher | Berlin : Nature Publishing | eng |
dc.relation.doi | https://doi.org/10.1038/s41467-019-12165-x | |
dc.rights.license | CC BY 4.0 Unported | eng |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | eng |
dc.subject.ddc | 620 | eng |
dc.subject.other | SR-SIM | eng |
dc.subject.other | microscopy | eng |
dc.subject.other | VIGOR | eng |
dc.title | Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction | eng |
dc.type | Article | eng |
dc.type | Text | eng |
tib.accessRights | openAccess | eng |
wgl.contributor | IPHT | eng |
wgl.subject | Ingenieurwissenschaften | eng |
wgl.type | Zeitschriftenartikel | eng |
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