Statistical Analysis of Scanning Fluorescence Correlation Spectroscopy Data Differentiates Free from Hindered Diffusion

dc.bibliographicCitation.firstPage8540
dc.bibliographicCitation.issue8
dc.bibliographicCitation.journalTitleACS nanoeng
dc.bibliographicCitation.lastPage8546
dc.bibliographicCitation.volume12
dc.contributor.authorSchneider, Falk
dc.contributor.authorWaithe, Dominic
dc.contributor.authorLagerholm, B. Christoffer
dc.contributor.authorShrestha, Dilip
dc.contributor.authorSezgin, Erdinc
dc.contributor.authorEggeling, Christian
dc.contributor.authorFritzsche, Marco
dc.date.accessioned2023-01-19T09:43:49Z
dc.date.available2023-01-19T09:43:49Z
dc.date.issued2018-7-20
dc.description.abstractCells rely on versatile diffusion dynamics in their plasma membrane. Quantification of this often heterogeneous diffusion is essential to the understanding of cell regulation and function. Yet such measurements remain a major challenge in cell biology, usually due to low sampling throughput, a necessity for dedicated equipment, sophisticated fluorescent label strategies, and limited sensitivity. Here, we introduce a robust, broadly applicable statistical analysis pipeline for large scanning fluorescence correlation spectroscopy data sets, which uncovers the nanoscale heterogeneity of the plasma membrane in living cells by differentiating free from hindered diffusion modes of fluorescent lipid and protein analogues.eng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/10921
dc.identifier.urihttp://dx.doi.org/10.34657/9947
dc.language.isoeng
dc.publisherWashington, DC : Soc.
dc.relation.doihttps://doi.org/10.1021/acsnano.8b04080
dc.relation.essn1936-086X
dc.relation.issn1936-0851
dc.rights.licenseCC BY 4.0 Unported
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.ddc540
dc.subject.otheractin cytoskeletoneng
dc.subject.otherBrownianeng
dc.subject.otherdiffusion modeseng
dc.subject.otherfree and trapped diffusioneng
dc.subject.otherhindered diffusion dynamicseng
dc.subject.otherscanning FCSeng
dc.titleStatistical Analysis of Scanning Fluorescence Correlation Spectroscopy Data Differentiates Free from Hindered Diffusioneng
dc.typeArticleeng
dc.typeTexteng
tib.accessRightsopenAccesseng
wgl.contributorIPHT
wgl.subjectChemieger
wgl.typeZeitschriftenartikelger
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Statistical_Analysis_of_Scanning.pdf
Size:
5.91 MB
Format:
Adobe Portable Document Format
Description:
Collections