Microfluidic-assisted silk nanoparticle tuning
dc.bibliographicCitation.firstPage | 873 | eng |
dc.bibliographicCitation.issue | 2 | eng |
dc.bibliographicCitation.journalTitle | Nanoscale advances | eng |
dc.bibliographicCitation.lastPage | 883 | eng |
dc.bibliographicCitation.volume | 1 | eng |
dc.contributor.author | Wongpinyochit, Thidarat | |
dc.contributor.author | Totten, John D. | |
dc.contributor.author | Johnston, Blair F. | |
dc.contributor.author | Seib, F. Philipp | |
dc.date.accessioned | 2021-11-26T07:33:15Z | |
dc.date.available | 2021-11-26T07:33:15Z | |
dc.date.issued | 2019 | |
dc.description.abstract | Silk is now making inroads into advanced pharmaceutical and biomedical applications. Both bottom-up and top-down approaches can be applied to silk and the resulting aqueous silk solution can be processed into a range of material formats, including nanoparticles. Here, we demonstrate the potential of microfluidics for the continuous production of silk nanoparticles with tuned particle characteristics. Our microfluidic-based design ensured efficient mixing of different solvent phases at the nanoliter scale, in addition to controlling the solvent ratio and flow rates. The total flow rate and aqueous : solvent ratios were important parameters affecting yield (1 mL min−1 > 12 mL min−1). The ratios also affected size and stability; a solvent : aqueous total flow ratio of 5 : 1 efficiently generated spherical nanoparticles 110 and 215 nm in size that were stable in water and had a high beta-sheet content. These 110 and 215 nm silk nanoparticles were not cytotoxic (IC50 > 100 μg mL−1) but showed size-dependent cellular trafficking. Overall, microfluidic-assisted silk nanoparticle manufacture is a promising platform that allows control of the silk nanoparticle properties by manipulation of the processing variables. | eng |
dc.description.version | publishedVersion | eng |
dc.identifier.uri | https://oa.tib.eu/renate/handle/123456789/7505 | |
dc.identifier.uri | https://doi.org/10.34657/6552 | |
dc.language.iso | eng | eng |
dc.publisher | Cambridge : Royal Society of Chemistry | eng |
dc.relation.doi | https://doi.org/10.1039/c8na00208h | |
dc.relation.essn | 2516-0230 | |
dc.rights.license | CC BY 3.0 Unported | eng |
dc.rights.uri | https://creativecommons.org/licenses/by/3.0/ | eng |
dc.subject.ddc | 540 | eng |
dc.subject.other | silk | eng |
dc.subject.other | silk nanoparticle | eng |
dc.subject.other | nanotechnology | eng |
dc.title | Microfluidic-assisted silk nanoparticle tuning | eng |
dc.type | Article | eng |
dc.type | Text | eng |
tib.accessRights | openAccess | eng |
wgl.contributor | IPF | eng |
wgl.subject | Chemie | eng |
wgl.type | Zeitschriftenartikel | eng |
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