Rolled-up functionalized nanomembranes as three-dimensional cavities for single cell studies

dc.bibliographicCitation.firstPage4197eng
dc.bibliographicCitation.issue8eng
dc.bibliographicCitation.journalTitleNano Letterseng
dc.bibliographicCitation.volume14eng
dc.contributor.authorXi, W.
dc.contributor.authorSchmidt, C.K.
dc.contributor.authorSanchez, S.
dc.contributor.authorGracias, D.H.
dc.contributor.authorCarazo-Salas, R.E.
dc.contributor.authorJackson, S.P.
dc.contributor.authorSchmidt, O.G.
dc.date.accessioned2020-11-12T07:22:04Z
dc.date.available2020-11-12T07:22:04Z
dc.date.issued2014
dc.description.abstractWe use micropatterning and strain engineering to encapsulate single living mammalian cells into transparent tubular architectures consisting of three-dimensional (3D) rolled-up nanomembranes. By using optical microscopy, we demonstrate that these structures are suitable for the scrutiny of cellular dynamics within confined 3D-microenvironments. We show that spatial confinement of mitotic mammalian cells inside tubular architectures can perturb metaphase plate formation, delay mitotic progression, and cause chromosomal instability in both a transformed and nontransformed human cell line. These findings could provide important clues into how spatial constraints dictate cellular behavior and function.eng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://doi.org/10.34657/4523
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/5894
dc.language.isoengeng
dc.publisherWashington, DC : American Chemical Societyeng
dc.relation.doihttps://doi.org/10.1021/nl4042565
dc.relation.issn1530-6984
dc.rights.licenseACS AuthorChoiceeng
dc.rights.urihttps://pubs.acs.org/page/policy/authorchoice_termsofuse.htmleng
dc.subject.ddc620eng
dc.subject.other3D cell culture scaffoldeng
dc.subject.otherchromosome segregation errorseng
dc.subject.othermitosiseng
dc.subject.otherNanomembraneseng
dc.subject.otherrolled-up nanotechnologyeng
dc.subject.otherspatial confinementeng
dc.subject.otherCell cultureeng
dc.subject.otherChromosomeseng
dc.subject.otherNanostructureseng
dc.subject.other3-D cell cultureeng
dc.subject.otherChromosome segregationeng
dc.subject.othermitosiseng
dc.subject.otherNanomembraneseng
dc.subject.otherSpatial confinementeng
dc.subject.otherScaffolds (biology)eng
dc.subject.otherartificial membraneeng
dc.subject.othernanomaterialeng
dc.subject.otherartificial membraneeng
dc.subject.otherchemistryeng
dc.subject.otherHeLa cell lineeng
dc.subject.otherhumaneng
dc.subject.othermetaphaseeng
dc.subject.otherHeLa Cellseng
dc.subject.otherHumanseng
dc.subject.otherMembranes, Artificialeng
dc.subject.otherMetaphaseeng
dc.subject.otherNanostructureseng
dc.titleRolled-up functionalized nanomembranes as three-dimensional cavities for single cell studieseng
dc.typeArticleeng
dc.typeTexteng
tib.accessRightsopenAccesseng
wgl.contributorIFWDeng
wgl.subjectIngenieurwissenschafteneng
wgl.typeZeitschriftenartikeleng
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