Towards a Biohybrid Lung: Endothelial Cells Promote Oxygen Transfer through Gas Permeable Membranes
dc.bibliographicCitation.firstPage | 5258196 | eng |
dc.bibliographicCitation.journalTitle | BioMed research international | eng |
dc.bibliographicCitation.volume | 2017 | eng |
dc.contributor.author | Menzel, Sarah | |
dc.contributor.author | Finocchiaro, Nicole | |
dc.contributor.author | Donay, Christine | |
dc.contributor.author | Thiebes, Anja Lena | |
dc.contributor.author | Hesselmann, Felix | |
dc.contributor.author | Arens, Jutta | |
dc.contributor.author | Djeljadini, Suzana | |
dc.contributor.author | Wessling, Matthias | |
dc.contributor.author | Schmitz-Rode, Thomas | |
dc.contributor.author | Jockenhoevel, Stefan | |
dc.contributor.author | Cornelissen, Christian Gabriel | |
dc.date.accessioned | 2022-03-18T10:38:13Z | |
dc.date.available | 2022-03-18T10:38:13Z | |
dc.date.issued | 2017 | |
dc.description.abstract | In patients with respiratory failure, extracorporeal lung support can ensure the vital gas exchange via gas permeable membranes but its application is restricted by limited long-term stability and hemocompatibility of the gas permeable membranes, which are in contact with the blood. Endothelial cells lining these membranes promise physiological hemocompatibility and should enable prolonged application. However, the endothelial cells increase the diffusion barrier of the blood-gas interface and thus affect gas transfer. In this study, we evaluated how the endothelial cells affect the gas exchange to optimize performance while maintaining an integral cell layer. Human umbilical vein endothelial cells were seeded on gas permeable cell culture membranes and cultivated in a custom-made bioreactor. Oxygen transfer rates of blank and endothelialized membranes in endothelial culture medium were determined. Cell morphology was assessed by microscopy and immunohistochemistry. Both setups provided oxygenation of the test fluid featuring small standard deviations of the measurements. Throughout the measuring range, the endothelial cells seem to promote gas transfer to a certain extent exceeding the blank membranes gas transfer performance by up to 120%. Although the underlying principles hereof still need to be clarified, the results represent a significant step towards the development of a biohybrid lung. | eng |
dc.description.version | publishedVersion | eng |
dc.identifier.uri | https://oa.tib.eu/renate/handle/123456789/8274 | |
dc.identifier.uri | https://doi.org/10.34657/7312 | |
dc.language.iso | eng | eng |
dc.publisher | New York, NY [u.a.] : Hindawi Publ. Corp. | eng |
dc.relation.doi | https://doi.org/10.1155/2017/5258196 | |
dc.relation.essn | 2314-6141 | |
dc.rights.license | CC BY 4.0 Unported | eng |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | eng |
dc.subject.ddc | 610 | eng |
dc.subject.ddc | 570 | eng |
dc.subject.other | Respiratory Physiological Phenomena | eng |
dc.subject.other | Pulmonary Gas Exchange | eng |
dc.subject.other | CD31 antigen | eng |
dc.subject.other | lung | eng |
dc.subject.other | Human Umbilical Vein Endothelial Cells | eng |
dc.subject.other | oxygen | eng |
dc.subject.other | Cell Line | eng |
dc.subject.other | respiratory function | eng |
dc.subject.other | lung gas exchange | eng |
dc.subject.other | cell membrane permeability | eng |
dc.subject.other | umbilical vein endothelial cell | eng |
dc.subject.other | von Willebrand factor | eng |
dc.subject.other | bioreactor | eng |
dc.subject.other | bright field microscopy | eng |
dc.subject.other | oxygenation | eng |
dc.subject.other | measurement accuracy | eng |
dc.subject.other | mathematical parameters | eng |
dc.subject.other | lung endothelium | eng |
dc.subject.other | lung diffusion | eng |
dc.subject.other | immunohistochemistry | eng |
dc.subject.other | cell culture | eng |
dc.subject.other | cell isolation | eng |
dc.subject.other | cell structure | eng |
dc.subject.other | endothelium cell | eng |
dc.subject.other | fluid flow | eng |
dc.subject.other | fluorescence microscopy | eng |
dc.subject.other | gas permeability | eng |
dc.title | Towards a Biohybrid Lung: Endothelial Cells Promote Oxygen Transfer through Gas Permeable Membranes | eng |
dc.type | Article | eng |
dc.type | Text | eng |
tib.accessRights | openAccess | eng |
wgl.contributor | DWI | eng |
wgl.subject | Medizin, Gesundheit | eng |
wgl.type | Zeitschriftenartikel | eng |
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