Periodic Exposure of Keratinocytes to Cold Physical Plasma: An In Vitro Model for Redox-Related Diseases of the Skin
dc.bibliographicCitation.firstPage | 9816072 | eng |
dc.bibliographicCitation.journalTitle | Oxidative medicine and cellular longevity | eng |
dc.bibliographicCitation.volume | 2016 | eng |
dc.contributor.author | Schmidt, Anke | |
dc.contributor.author | von Woedtke, Thomas | |
dc.contributor.author | Bekeschus, Sander | |
dc.date.accessioned | 2022-07-06T07:18:20Z | |
dc.date.available | 2022-07-06T07:18:20Z | |
dc.date.issued | 2016 | |
dc.description.abstract | Oxidative stress illustrates an imbalance between radical formation and removal. Frequent redox stress is critically involved in many human pathologies including cancer, psoriasis, and chronic wounds. However, reactive species pursue a dual role being involved in signaling on the one hand and oxidative damage on the other. Using a HaCaT keratinocyte cell culture model, we investigated redox regulation and inflammation to periodic, low-dose oxidative stress after two, six, eight, ten, and twelve weeks. Chronic redox stress was generated by recurrent incubation with cold physical plasma-treated cell culture medium. Using transcriptome microarray technology, we identified both acute ROS-stress responses as well as numerous adaptions after several weeks of redox challenge. We determined a differential expression (2-fold, FDR < 0.01, p < 0.05) of 260 genes that function in inflammation and redox homeostasis, such as cytokines (e.g., IL-6, IL-8, and IL-10), growth factors (e.g., CSF2, FGF, and IGF-2), and antioxidant enzymes (e.g., HMOX, NQO1, GPX, and PRDX). Apoptotic signaling was affected rather modestly, especially in p53 downstream targets (e.g., BCL2, BBC3, and GADD45). Strikingly, the cell-protective heat shock protein HSP27 was strongly upregulated (p < 0.001). These results suggested cellular adaptions to frequent redox stress and may help to better understand the inflammatory responses in redox-related diseases. | eng |
dc.description.version | publishedVersion | eng |
dc.identifier.uri | https://oa.tib.eu/renate/handle/123456789/9634 | |
dc.identifier.uri | https://doi.org/10.34657/8672 | |
dc.language.iso | eng | eng |
dc.publisher | London: Hindawi | eng |
dc.relation.doi | https://doi.org/10.1155/2016/9816072 | |
dc.relation.essn | 1942-0994 | |
dc.rights.license | CC BY 4.0 Unported | eng |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | eng |
dc.subject.ddc | 610 | eng |
dc.subject.other | Gene expression | eng |
dc.subject.other | Pathology | eng |
dc.subject.other | Antioxidant enzyme | eng |
dc.subject.other | Apoptotic signaling | eng |
dc.subject.other | Cell culture mediums | eng |
dc.subject.other | Cell culture models | eng |
dc.subject.other | Heat shock protein | eng |
dc.subject.other | Inflammatory response | eng |
dc.subject.other | Microarray technologies | eng |
dc.subject.other | Radical formation | eng |
dc.subject.other | Cell culture | eng |
dc.subject.other | antioxidant | eng |
dc.subject.other | cytokine | eng |
dc.subject.other | oxidoreductase | eng |
dc.subject.other | plasma gas | eng |
dc.subject.other | reactive oxygen metabolite | eng |
dc.subject.other | adverse effects | eng |
dc.subject.other | biological model | eng |
dc.subject.other | biosynthesis | eng |
dc.subject.other | cell line | eng |
dc.subject.other | gene expression regulation | eng |
dc.subject.other | human | eng |
dc.subject.other | keratinocyte | eng |
dc.subject.other | metabolism | eng |
dc.subject.other | oxidation reduction reaction | eng |
dc.subject.other | pathology | eng |
dc.subject.other | plasma gas | eng |
dc.subject.other | Skin Diseases | eng |
dc.subject.other | Antioxidants | eng |
dc.subject.other | Cell Line | eng |
dc.subject.other | Cytokines | eng |
dc.subject.other | Gene Expression Regulation | eng |
dc.subject.other | Humans | eng |
dc.subject.other | Keratinocytes | eng |
dc.subject.other | Models, Biological | eng |
dc.subject.other | Oxidation-Reduction | eng |
dc.subject.other | Oxidoreductases | eng |
dc.subject.other | Plasma Gases | eng |
dc.subject.other | Reactive Oxygen Species | eng |
dc.subject.other | Skin Diseases | eng |
dc.title | Periodic Exposure of Keratinocytes to Cold Physical Plasma: An In Vitro Model for Redox-Related Diseases of the Skin | eng |
dc.type | Article | eng |
dc.type | Text | eng |
tib.accessRights | openAccess | eng |
wgl.contributor | INP | eng |
wgl.subject | Medizin, Gesundheit | eng |
wgl.type | Zeitschriftenartikel | eng |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- Periodic_Exposure_of_Keratinocyte.pdf
- Size:
- 5.2 MB
- Format:
- Adobe Portable Document Format
- Description: