Protein O-mannosylation in the murine brain: Occurrence of Mono-O-Mannosyl glycans and identification of new substrates
dc.bibliographicCitation.issue | 11 | eng |
dc.bibliographicCitation.journalTitle | PLOS ONE | eng |
dc.bibliographicCitation.volume | 11 | |
dc.contributor.author | Bartels, Markus F. | |
dc.contributor.author | Winterhalter, Patrick R. | |
dc.contributor.author | Yu, Jin | |
dc.contributor.author | Liu, Yan | |
dc.contributor.author | Lommel, Mark | |
dc.contributor.author | Möhrlen, Frank | |
dc.contributor.author | Hu, Huaiyu | |
dc.contributor.author | Feizi, Ten | |
dc.contributor.author | Westerlind, Ulrika | |
dc.contributor.author | Ruppert, Thomas | |
dc.contributor.author | Strahl, Sabine | |
dc.date.accessioned | 2018-02-20T22:04:50Z | |
dc.date.available | 2019-06-26T17:18:34Z | |
dc.date.issued | 2016 | |
dc.description.abstract | Protein O-mannosylation is a post-translational modification essential for correct development of mammals. In humans, deficient O-mannosylation results in severe congenital muscular dystrophies often associated with impaired brain and eye development. Although various O-mannosylated proteins have been identified in the recent years, the distribution of O-mannosyl glycans in the mammalian brain and target proteins are still not well defined. In the present study, rabbit monoclonal antibodies directed against the O-mannosylated peptide YAT(α1-Man)AV were generated. Detailed characterization of clone RKU-1-3-5 revealed that this monoclonal antibody recognizes O-linked mannose also in different peptide and protein contexts. Using this tool, we observed that mono-O-mannosyl glycans occur ubiquitously throughout the murine brain but are especially enriched at inhibitory GABAergic neurons and at the perineural nets. Using a mass spectrometry-based approach, we further identified glycoproteins from the murine brain that bear single O-mannose residues. Among the candidates identified are members of the cadherin and plexin superfamilies and the perineural net protein neurocan. In addition, we identified neurexin 3, a cell adhesion protein involved in synaptic plasticity, and inter-alpha-trypsin inhibitor 5, a protease inhibitor important in stabilizing the extracellular matrix, as new O-mannosylated glycoproteins. | |
dc.description.version | publishedVersion | eng |
dc.format | application/pdf | |
dc.identifier.uri | https://doi.org/10.34657/1194 | |
dc.identifier.uri | https://oa.tib.eu/renate/handle/123456789/581 | |
dc.language.iso | eng | eng |
dc.publisher | San Francisco, CA : Public Library of Science | |
dc.relation.doi | https://doi.org/10.1371/journal.pone.0166119 | |
dc.rights.license | CC BY 4.0 Unported | eng |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | eng |
dc.subject.ddc | 550 | |
dc.subject.other | cadherin | eng |
dc.subject.other | calbindin | eng |
dc.subject.other | glycan derivative | eng |
dc.subject.other | mono o mannosyl glycan derivative | eng |
dc.subject.other | monoclonal antibody | eng |
dc.subject.other | neurexin | eng |
dc.subject.other | neurexin 3 | eng |
dc.subject.other | neurocan | eng |
dc.subject.other | plexin | eng |
dc.subject.other | unclassified drug | eng |
dc.subject.other | 4 aminobutyric acid receptor | eng |
dc.subject.other | glycoprotein | eng |
dc.subject.other | mannose | eng |
dc.subject.other | polysaccharide | eng |
dc.title | Protein O-mannosylation in the murine brain: Occurrence of Mono-O-Mannosyl glycans and identification of new substrates | |
dc.type | Article | eng |
dc.type | Text | eng |
tib.accessRights | openAccess | eng |
wgl.contributor | ISAS | eng |
wgl.subject | Geowissenschaften | eng |
wgl.type | Zeitschriftenartikel | eng |
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