Phenotypic, Morphological and Adhesive Differences of Human Hematopoietic Progenitor Cells Cultured on Murine versus Human Mesenchymal Stromal Cells

dc.bibliographicCitation.firstPage15680
dc.bibliographicCitation.journalTitleScientific reportseng
dc.bibliographicCitation.volume5
dc.contributor.authorReichert, Doreen
dc.contributor.authorFriedrichs, Jens
dc.contributor.authorRitter, Steffi
dc.contributor.authorKäubler, Theresa
dc.contributor.authorWerner, Carsten
dc.contributor.authorBornhäuser, Martin
dc.contributor.authorCorbeil, Denis
dc.date.accessioned2022-06-01T05:33:09Z
dc.date.available2022-06-01T05:33:09Z
dc.date.issued2015
dc.description.abstractXenogenic transplantation models have been developed to study human hematopoiesis in immunocompromised murine recipients. They still have limitations and therefore it is important to delineate all players within the bone marrow that could account for species-specific differences. Here, we evaluated the proliferative capacity, morphological and physical characteristics of human CD34+ hematopoietic stem and progenitor cells (HSPCs) after co-culture on murine or human bone marrow-derived mesenchymal stromal cells (MSCs). After seven days, human CD34+CD133– HSPCs expanded to similar extents on both feeder layers while cellular subsets comprising primitive CD34+CD133+ and CD133+CD34– phenotypes are reduced fivefold on murine MSCs. The number of migrating HSPCs was also reduced on murine cells suggesting that MSC adhesion influences cellular polarization of HSPC. We used atomic force microscopy-based single-cell force spectroscopy to quantify their adhesive interactions. We found threefold higher detachment forces of human HSPCs from murine MSCs compared to human ones. This difference is related to the N-cadherin expression level on murine MSCs since its knockdown abolished their differential adhesion properties with human HSPCs. Our observations highlight phenotypic, morphological and adhesive differences of human HSPCs when cultured on murine or human MSCs, which raise some caution in data interpretation when xenogenic transplantation models are used.eng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/9043
dc.identifier.urihttps://doi.org/10.34657/8081
dc.language.isoengeng
dc.publisher[London] : Macmillan Publishers Limited, part of Springer Nature
dc.relation.doihttps://doi.org/10.1038/srep15680
dc.relation.essn2045-2322
dc.rights.licenseCC BY 4.0 Unported
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.ddc500
dc.subject.ddc600
dc.subject.meshAC133 Antigeneng
dc.subject.meshAnimalseng
dc.subject.meshAntigens, CDeng
dc.subject.meshAntigens, CD34eng
dc.subject.meshCadherinseng
dc.subject.meshCell Adhesioneng
dc.subject.meshCell Differentiationeng
dc.subject.meshCell Movementeng
dc.subject.meshCell Polarityeng
dc.subject.meshCells, Culturedeng
dc.subject.meshCoculture Techniqueseng
dc.subject.meshGlycoproteinseng
dc.subject.meshHematopoiesiseng
dc.subject.meshHematopoietic Stem Cellseng
dc.subject.meshHumanseng
dc.subject.meshMesenchymal Stromal Cellseng
dc.subject.meshMiceeng
dc.subject.meshMice, Inbred C57BLeng
dc.subject.meshMicroscopy, Atomic Forceeng
dc.subject.meshPeptideseng
dc.subject.meshPhenotypeeng
dc.subject.meshRNA Interferenceeng
dc.subject.meshTransport Vesicleseng
dc.subject.othercadherineng
dc.subject.otherCD133 antigeneng
dc.subject.otherCD34 antigeneng
dc.subject.otherglycoproteineng
dc.subject.otherleukocyte antigeneng
dc.subject.otherpeptideeng
dc.subject.otherPROM1 protein, humaneng
dc.subject.otherProm1 protein, mouseeng
dc.subject.otheranimaleng
dc.subject.otherantagonists and inhibitorseng
dc.subject.otheratomic force microscopyeng
dc.subject.otherC57BL mouseeng
dc.subject.othercell adhesioneng
dc.subject.othercell cultureeng
dc.subject.othercell differentiationeng
dc.subject.othercell motioneng
dc.subject.othercell polarityeng
dc.subject.othercocultureeng
dc.subject.othercytologyeng
dc.subject.othergeneticseng
dc.subject.otherhematopoiesiseng
dc.subject.otherhematopoietic stem celleng
dc.subject.otherhumaneng
dc.subject.othermesenchymal stroma celleng
dc.subject.othermetabolismeng
dc.subject.othermouseeng
dc.subject.otherphenotypeeng
dc.subject.otherRNA interferenceeng
dc.subject.othertransport vesicleeng
dc.titlePhenotypic, Morphological and Adhesive Differences of Human Hematopoietic Progenitor Cells Cultured on Murine versus Human Mesenchymal Stromal Cellseng
dc.typeArticleeng
dc.typeTexteng
tib.accessRightsopenAccesseng
wgl.contributorIPFger
wgl.subjectMedizin, Gesundheitger
wgl.subjectBiowissenschaften/Biologieger
wgl.typeZeitschriftenartikelger
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