Blind Super-Resolution Approach for Exploiting Illumination Variety in Optical-Lattice Illumination Microscopy

Loading...
Thumbnail Image
Date
2021
Volume
8
Issue
9
Journal
ACS Photonics
Series Titel
Book Title
Publisher
Washington, DC : ACS Publications
Abstract

Optical-lattice illumination patterns help in pushing high spatial frequency components of the sample into the optical transfer function of a collection microscope. However, exploiting these high-frequency components require precise knowledge of illumination if reconstruction approaches similar to structured illumination microscopy are employed. Here, we present an alternate blind reconstruction approach that can provide super-resolution without the requirement of extra frames. For this, the property of exploiting temporal fluctuations in the sample emissions using “multiple signal classification algorithm” is extended aptly toward using spatial fluctuation of phase-modulated lattice illuminations for super-resolution. The super-resolution ability is shown for sinusoidal and multiperiodic lattice with approximately 3- and 6-fold resolution enhancements, respectively, over the diffraction limit. © 2021 The Authors. Published by American Chemical Society

Description
Keywords
Citation
Samanta, K., Sarkar, S., Acuña, S., Joseph, J., Ahluwalia, B. S., & Agarwal, K. (2021). Blind Super-Resolution Approach for Exploiting Illumination Variety in Optical-Lattice Illumination Microscopy (Washington, DC : ACS Publications). Washington, DC : ACS Publications. https://doi.org//10.1021/acsphotonics.1c00503
License
CC BY-NC-ND 4.0 Unported