Granular Cellulose Nanofibril Hydrogel Scaffolds for 3D Cell Cultivation

dc.bibliographicCitation.firstPage2000191eng
dc.bibliographicCitation.issue18eng
dc.bibliographicCitation.volume41eng
dc.contributor.authorGehlen, David B.
dc.contributor.authorJürgens, Niklas
dc.contributor.authorOmidinia-Anarkoli, Abdolrahman
dc.contributor.authorHaraszti, Tamás
dc.contributor.authorGeorge, Julian
dc.contributor.authorWalther, Andreas
dc.contributor.authorYe, Hua
dc.contributor.authorDe Laporte, Laura
dc.date.accessioned2021-08-03T06:59:27Z
dc.date.available2021-08-03T06:59:27Z
dc.date.issued2020
dc.description.abstractThe replacement of diseased and damaged organs remains an challenge in modern medicine. However, through the use of tissue engineering techniques, it may soon be possible to (re)generate tissues and organs using artificial scaffolds. For example, hydrogel networks made from hydrophilic precursor solutions can replicate many properties found in the natural extracellular matrix (ECM) but often lack the dynamic nature of the ECM, as many covalently crosslinked hydrogels possess elastic and static networks with nanoscale pores hindering cell migration without being degradable. To overcome this, macroporous colloidal hydrogels can be prepared to facilitate cell infiltration. Here, an easy method is presented to fabricate granular cellulose nanofibril hydrogel (CNF) scaffolds as porous networks for 3D cell cultivation. CNF is an abundant natural and highly biocompatible material that supports cell adhesion. Granular CNF scaffolds are generated by pre-crosslinking CNF using calcium and subsequently pressing the gel through micrometer-sized nylon meshes. The granular solution is mixed with fibroblasts and crosslinked with cell culture medium. The obtained granular CNF scaffold is significantly softer and enables well-distributed fibroblast growth. This cost-effective material combined with this efficient and facile fabrication technique allows for 3D cell cultivation in an upscalable manner. © 2020 The Authors. Published by Wiley-VCH GmbHeng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/6466
dc.identifier.urihttps://doi.org/10.34657/5513
dc.language.isoengeng
dc.publisherWeinheim : Wiley-VCHeng
dc.relation.doihttps://doi.org/10.1002/marc.202000191
dc.relation.essn1521-3927
dc.relation.isbn1022-1336
dc.relation.ispartofseriesMacromolecular Rapid Communications 41 (2020), Nr. 18eng
dc.relation.issn0173-2803
dc.rights.licenseCC BY-NC-ND 4.0 Unportedeng
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/eng
dc.subject3D cell cultureseng
dc.subjectcellulose nanofibril hydrogelseng
dc.subjectgranular hydrogel scaffoldseng
dc.subjecttissue engineeringeng
dc.subject.ddc540eng
dc.titleGranular Cellulose Nanofibril Hydrogel Scaffolds for 3D Cell Cultivationeng
dc.typearticleeng
dc.typeTexteng
dcterms.bibliographicCitation.journalTitleMacromolecular Rapid Communicationseng
tib.accessRightsopenAccesseng
wgl.contributorDWIeng
wgl.subjectChemieeng
wgl.typeZeitschriftenartikeleng
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