2D polarization imaging as a low-cost fluorescence method to detect α-synuclein aggregation ex vivo in models of Parkinson’s disease
dc.bibliographicCitation.firstPage | 157 | eng |
dc.bibliographicCitation.lastPage | 280 | eng |
dc.bibliographicCitation.volume | 1 | eng |
dc.contributor.author | Camacho, Rafael | |
dc.contributor.author | Täuber, Daniela | |
dc.contributor.author | Hansen, Christian | |
dc.contributor.author | Shi, Juanzi | |
dc.contributor.author | Bousset, Luc | |
dc.contributor.author | Melki, Ronald | |
dc.contributor.author | Li, Jia-Yi | |
dc.contributor.author | Scheblykin, Ivan G. | |
dc.date.accessioned | 2020-01-03T10:09:19Z | |
dc.date.available | 2020-01-03T10:09:19Z | |
dc.date.issued | 2018 | |
dc.description.abstract | A hallmark of Parkinson’s disease is the formation of large protein-rich aggregates in neurons, where α-synuclein is the most abundant protein. A standard approach to visualize aggregation is to fluorescently label the proteins of interest. Then, highly fluorescent regions are assumed to contain aggregated proteins. However, fluorescence brightness alone cannot discriminate micrometer-sized regions with high expression of non-aggregated proteins from regions where the proteins are aggregated on the molecular scale. Here, we demonstrate that 2-dimensional polarization imaging can discriminate between preformed non-aggregated and aggregated forms of α-synuclein, and detect increased aggregation in brain tissues of transgenic mice. This imaging method assesses homo-FRET between labels by measuring fluorescence polarization in excitation and emission simultaneously, which translates into higher contrast than fluorescence anisotropy imaging. Exploring earlier aggregation states of α-synuclein using such technically simple imaging method could lead to crucial improvements in our understanding of α-synuclein-mediated pathology in Parkinson’s Disease. | eng |
dc.description.version | publishedVersion | eng |
dc.identifier.uri | https://doi.org/10.34657/7 | |
dc.identifier.uri | https://oa.tib.eu/renate/handle/123456789/4736 | |
dc.language.iso | eng | eng |
dc.publisher | Berlin : Nature Publishing | eng |
dc.relation.doi | https://doi.org/10.1038/s42003-018-0156-x | |
dc.relation.ispartofseries | Communications Biology 1 (2018) | eng |
dc.rights.license | CC BY 4.0 Unported | eng |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | eng |
dc.subject | Parkinson’s disease | eng |
dc.subject | protein-rich aggregates | eng |
dc.subject | α-synuclein | eng |
dc.subject.ddc | 570 | eng |
dc.title | 2D polarization imaging as a low-cost fluorescence method to detect α-synuclein aggregation ex vivo in models of Parkinson’s disease | eng |
dc.type | article | eng |
dc.type | Text | eng |
dcterms.bibliographicCitation.journalTitle | Communications Biology | eng |
tib.accessRights | openAccess | eng |
wgl.contributor | IPHT | eng |
wgl.subject | Ingenieurwissenschaften | eng |
wgl.type | Zeitschriftenartikel | eng |
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