Biochemical Analysis of Leukocytes after In Vitro and In Vivo Activation with Bacterial and Fungal Pathogens Using Raman Spectroscopy

dc.bibliographicCitation.firstPage10481
dc.bibliographicCitation.issue19
dc.bibliographicCitation.journalTitleInternational Journal of Molecular Scienceseng
dc.bibliographicCitation.volume22
dc.contributor.authorPistiki, Aikaterini
dc.contributor.authorRamoji, Anuradha
dc.contributor.authorRyabchykov, Oleg
dc.contributor.authorThomas-Rueddel, Daniel
dc.contributor.authorPress, Adrian T.
dc.contributor.authorMakarewicz, Oliwia
dc.contributor.authorGiamarellos-Bourboulis, Evangelos J.
dc.contributor.authorBauer, Michael
dc.contributor.authorBocklitz, Thomas
dc.contributor.authorPopp, Juergen
dc.contributor.authorNeugebauer, Ute
dc.date.accessioned2022-03-31T11:27:37Z
dc.date.available2022-03-31T11:27:37Z
dc.date.issued2021
dc.description.abstractBiochemical information from activated leukocytes provide valuable diagnostic information. In this study, Raman spectroscopy was applied as a label-free analytical technique to characterize the activation pattern of leukocyte subpopulations in an in vitro infection model. Neutrophils, monocytes, and lymphocytes were isolated from healthy volunteers and stimulated with heat-inactivated clinical isolates of Candida albicans, Staphylococcus aureus, and Klebsiella pneumoniae. Binary classification models could identify the presence of infection for monocytes and lymphocytes, classify the type of infection as bacterial or fungal for neutrophils, monocytes, and lymphocytes and distinguish the cause of infection as Gram-negative or Gram-positive bacteria in the monocyte subpopulation. Changes in single-cell Raman spectra, upon leukocyte stimulation, can be explained with biochemical changes due to the leukocyte’s specific reaction to each type of pathogen. Raman spectra of leukocytes from the in vitro infection model were compared with spectra from leukocytes of patients with infection (DRKS-ID: DRKS00006265) with the same pathogen groups, and a good agreement was revealed. Our study elucidates the potential of Raman spectroscopy-based single-cell analysis for the differentiation of circulating leukocyte subtypes and identification of the infection by probing the molecular phenotype of those cells.eng
dc.description.fondsLeibniz_Fonds
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/8493
dc.identifier.urihttps://doi.org/10.34657/7531
dc.language.isoeng
dc.publisherBasel : MDPI
dc.relation.doihttps://doi.org/10.3390/ijms221910481
dc.relation.essn1422-0067
dc.relation.issn1661-6596
dc.rights.licenseCC BY 4.0 Unported
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.ddc570eng
dc.subject.ddc540eng
dc.subject.otherCandida albicanseng
dc.subject.otherInfection modeleng
dc.subject.otherKlebsiella pneumoniaeeng
dc.subject.otherLeukocyteseng
dc.subject.otherLymphocyteeng
dc.subject.otherMonocyteeng
dc.subject.otherNeutrophileng
dc.subject.otherPBMCeng
dc.subject.otherRaman microspectroscopyeng
dc.subject.otherStaphylococcus aureuseng
dc.titleBiochemical Analysis of Leukocytes after In Vitro and In Vivo Activation with Bacterial and Fungal Pathogens Using Raman Spectroscopyeng
dc.typeArticleeng
dc.typeTexteng
tib.accessRightsopenAccess
wgl.contributorIPHT
wgl.subjectBiowissenschaften/Biologie
wgl.subjectChemie
wgl.typeZeitschriftenartikel
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