Adaptive optics allows STED-FCS measurements in the cytoplasm of living cells
dc.bibliographicCitation.firstPage | 23378 | eng |
dc.bibliographicCitation.issue | 16 | eng |
dc.bibliographicCitation.journalTitle | Optics Express | eng |
dc.bibliographicCitation.lastPage | 6 | eng |
dc.bibliographicCitation.volume | 27 | eng |
dc.contributor.author | Barbotin, Aurélien | |
dc.contributor.author | Galiani, Silvia | |
dc.contributor.author | Urbančič, Iztok | |
dc.contributor.author | Eggeling, Christian | |
dc.contributor.author | Booth, Martin J. | |
dc.date.accessioned | 2020-01-03T14:03:29Z | |
dc.date.available | 2020-01-03T14:03:29Z | |
dc.date.issued | 2019 | |
dc.description.abstract | Fluorescence correlation spectroscopy in combination with super-resolution stimulated emission depletion microscopy (STED-FCS) is a powerful tool to investigate molecular diffusion with sub-diffraction resolution. It has been of particular use for investigations of two dimensional systems like cell membranes, but has so far seen very limited applications to studies of three-dimensional diffusion. One reason for this is the extreme sensitivity of the axial (z) STED depletion pattern to optical aberrations. We present here an adaptive optics-based correction method that compensates for these aberrations and allows STED-FCS measurements in the cytoplasm of living cells. | eng |
dc.description.version | publishedVersion | eng |
dc.identifier.uri | https://doi.org/10.34657/69 | |
dc.identifier.uri | https://oa.tib.eu/renate/handle/123456789/4798 | |
dc.language.iso | eng | eng |
dc.publisher | Washington D.C. : Optical Society of America | eng |
dc.relation.doi | https://doi.org/10.1364/OE.27.023378 | |
dc.rights.license | CC BY 4.0 Unported | eng |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | eng |
dc.subject.ddc | 620 | eng |
dc.subject.other | STED-FCS | eng |
dc.subject.other | molecular diffusion | eng |
dc.subject.other | cells | eng |
dc.title | Adaptive optics allows STED-FCS measurements in the cytoplasm of living cells | eng |
dc.type | Article | eng |
dc.type | Text | eng |
tib.accessRights | openAccess | eng |
wgl.contributor | IPHT | eng |
wgl.subject | Ingenieurwissenschaften | eng |
wgl.type | Zeitschriftenartikel | eng |
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