Studying dynamic processes of nano-sized objects in liquid using scanning transmission electron microscopy
dc.bibliographicCitation.issue | 120 | eng |
dc.bibliographicCitation.journalTitle | Journal of Visualized Experiments (JoVE) | eng |
dc.contributor.author | de Jong, Niels | |
dc.contributor.author | Hermannsdörfer, Justus | |
dc.date.accessioned | 2018-04-04T09:13:11Z | |
dc.date.available | 2019-06-28T14:00:24Z | |
dc.date.issued | 2017 | |
dc.description.abstract | Samples fully embedded in liquid can be studied at a nanoscale spatial resolution with Scanning Transmission Electron Microscopy (STEM) using a microfluidic chamber assembled in the specimen holder for Transmission Electron Microscopy (TEM) and STEM. The microfluidic system consists of two silicon microchips supporting thin Silicon Nitride (SiN) membrane windows. This article describes the basic steps of sample loading and data acquisition. Most important of all is to ensure that the liquid compartment is correctly assembled, thus providing a thin liquid layer and a vacuum seal. This protocol also includes a number of tests necessary to perform during sample loading in order to ensure correct assembly. Once the sample is loaded in the electron microscope, the liquid thickness needs to be measured. Incorrect assembly may result in a too-thick liquid, while a too-thin liquid may indicate the absence of liquid, such as when a bubble is formed. Finally, the protocol explains how images are taken and how dynamic processes can be studied. A sample containing AuNPs is imaged both in pure water and in saline. | eng |
dc.description.version | publishedVersion | eng |
dc.format | application/pdf | |
dc.identifier.uri | https://doi.org/10.34657/1691 | |
dc.identifier.uri | https://oa.tib.eu/renate/handle/123456789/4670 | |
dc.language.iso | eng | eng |
dc.publisher | Cambridge : JoVE | eng |
dc.relation.doi | https://doi.org/10.3791/54943 | |
dc.rights.license | This document may be downloaded, read, stored and printed for your own use within the limits of § 53 UrhG but it may not be distributed via the internet or passed on to external parties. | eng |
dc.rights.license | Dieses Dokument darf im Rahmen von § 53 UrhG zum eigenen Gebrauch kostenfrei heruntergeladen, gelesen, gespeichert und ausgedruckt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. | ger |
dc.subject.ddc | 530 | eng |
dc.subject.other | Engineering | eng |
dc.subject.other | Issue 120 | eng |
dc.subject.other | Scanning transmission electron microscopy | eng |
dc.subject.other | STEM | eng |
dc.subject.other | liquid-phase STEM | eng |
dc.subject.other | dynamic processes | eng |
dc.subject.other | gold nanoparticle | eng |
dc.subject.other | microchip | eng |
dc.subject.other | silicon nitride membrane | eng |
dc.title | Studying dynamic processes of nano-sized objects in liquid using scanning transmission electron microscopy | eng |
dc.type | Article | eng |
dc.type | Text | eng |
tib.accessRights | openAccess | eng |
wgl.contributor | INM | eng |
wgl.subject | Physik | eng |
wgl.type | Zeitschriftenartikel | eng |
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