Cryo-EM structure of haemoglobin at 3.2 Å determined with the Volta phase plate

dc.bibliographicCitation.articleNumber16099
dc.bibliographicCitation.firstPage16099
dc.bibliographicCitation.issue1
dc.bibliographicCitation.journalTitleNature Communications
dc.bibliographicCitation.volume8
dc.contributor.authorKhoshouei, Maryam
dc.contributor.authorRadjainia, Mazdak
dc.contributor.authorBaumeister, Wolfgang
dc.contributor.authorDanev, Radostin
dc.date.accessioned2025-02-27T12:55:45Z
dc.date.available2025-02-27T12:55:45Z
dc.date.issued2017
dc.description.abstractWith the advent of direct electron detectors, the perspectives of cryo-electron microscopy (cryo-EM) have changed in a profound way. These cameras are superior to previous detectors in coping with the intrinsically low contrast and beam-induced motion of radiation-sensitive organic materials embedded in amorphous ice, and hence they have enabled the structure determination of many macromolecular assemblies to atomic or near-atomic resolution. Nevertheless, there are still limitations and one of them is the size of the target structure. Here, we report the use of a Volta phase plate in determining the structure of human haemoglobin (64 kDa) at 3.2 Å. Our results demonstrate that this method can be applied to complexes that are significantly smaller than those previously studied by conventional defocus-based approaches. Cryo-EM is now close to becoming a fast and cost-effective alternative to crystallography for high-resolution protein structure determination.eng
dc.description.versionpublishedVersioneng
dc.identifier.urihttps://oa.tib.eu/renate/handle/123456789/18647
dc.identifier.urihttps://doi.org/10.34657/17666
dc.language.isoeng
dc.publisher[London] : Springer Nature
dc.relation.doihttps://doi.org/10.1038/ncomms16099
dc.relation.essn2041-1723
dc.rights.licenseCC BY 4.0 Unported
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.subject.ddc500
dc.subject.otherCryoelectron Microscopyeng
dc.subject.otherHemoglobinseng
dc.subject.otherHumanseng
dc.subject.otherMolecular Structureeng
dc.subject.otherhemoglobineng
dc.subject.otherhemoglobineng
dc.subject.otheramorphous mediumeng
dc.subject.othercomplexityeng
dc.subject.othercrystallographyeng
dc.subject.otherelectron microscopyeng
dc.subject.otherequipmenteng
dc.subject.otherinduced responseeng
dc.subject.otherproteineng
dc.subject.otherArticleeng
dc.subject.otheratomeng
dc.subject.othercontrolled studyeng
dc.subject.othercost effectiveness analysiseng
dc.subject.othercryoelectron microscopyeng
dc.subject.othercrystallographyeng
dc.subject.otherhumaneng
dc.subject.othermacromoleculeeng
dc.subject.othermolecular sizeeng
dc.subject.othermotioneng
dc.subject.otherprotein determinationeng
dc.subject.otherprotein structureeng
dc.subject.otherradiosensitivityeng
dc.subject.otherchemical structureeng
dc.subject.othercryoelectron microscopyeng
dc.subject.otherevaluation studyeng
dc.subject.otherprocedureseng
dc.subject.otherultrastructureeng
dc.titleCryo-EM structure of haemoglobin at 3.2 Å determined with the Volta phase plateeng
dc.typeArticle
dc.typeText
tib.accessRightsopenAccess
wgl.contributorINP
wgl.subjectPhysikger
wgl.typeZeitschriftenartikelger
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