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Now showing 1 - 5 of 5
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    Characterization of Bathyarchaeota genomes assembled from metagenomes of biofilms residing in mesophilic and thermophilic biogas reactors
    (London : BioMed Central Ltd., 2018) Maus, I.; Rumming, M.; Bergmann, I.; Heeg, K.; Pohl, M.; Nettmann, E.; Jaenicke, S.; Blom, J.; Pühler, A.; Schlüter, A.; Sczyrba, A.; Klocke, M.
    Background: Previous studies on the Miscellaneous Crenarchaeota Group, recently assigned to the novel archaeal phylum Bathyarchaeota, reported on the dominance of these Archaea within the anaerobic carbohydrate cycle performed by the deep marine biosphere. For the first time, members of this phylum were identified also in mesophilic and thermophilic biogas-forming biofilms and characterized in detail. Results: Metagenome shotgun libraries of biofilm microbiomes were sequenced using the Illumina MiSeq system. Taxonomic classification revealed that between 0.1 and 2% of all classified sequences were assigned to Bathyarchaeota. Individual metagenome assemblies followed by genome binning resulted in the reconstruction of five metagenome-assembled genomes (MAGs) of Bathyarchaeota. MAGs were estimated to be 65-92% complete, ranging in their genome sizes from 1.1 to 2.0 Mb. Phylogenetic classification based on core gene sets confirmed their placement within the phylum Bathyarchaeota clustering as a separate group diverging from most of the recently known Bathyarchaeota clusters. The genetic repertoire of these MAGs indicated an energy metabolism based on carbohydrate and amino acid fermentation featuring the potential for extracellular hydrolysis of cellulose, cellobiose as well as proteins. In addition, corresponding transporter systems were identified. Furthermore, genes encoding enzymes for the utilization of carbon monoxide and/or carbon dioxide via the Wood-Ljungdahl pathway were detected. Conclusions: For the members of Bathyarchaeota detected in the biofilm microbiomes, a hydrolytic lifestyle is proposed. This is the first study indicating that Bathyarchaeota members contribute presumably to hydrolysis and subsequent fermentation of organic substrates within biotechnological biogas production processes.
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    Zielflächenorientierte, präzise Echtzeit-Fungizidapplikation in Getreide
    (Darmstadt : KTBL, 2015) Dammer, Karl-Heinz; Hamdorf, André; Ustyuzhanin, Anton; Schirrmann, Michael; Leithold, Peer; Leithold, Hermann; Volk, Thomas; Tackenberg, Maria
    Im Rahmen eines Verbundprojektes wurden Echtzeit-Applikationstechnologien mit berührungslosen Sensoren für präzise Fungizid-Spritzungen in Getreide entwickelt. Das Entscheidungshilfe- System proPlant expert.classic bzw. die Internetversion proPlant expert.com (proPlant GmbH) empfiehlt geeignete Fungizide und Dosierungen für ein bestimmtes Infektionsszenario der acht wichtigsten Blatt- und Ährenkrankheiten von Winterweizen. Das Precision- Farming-Modul „Fungizid“, welches auf dem Terminal in der Traktorenkabine läuft, steuert das präzise Spritzverfahren. Das Modul bestimmt die lokale Zielapplikationsmenge während des Spritzens durch Nutzung des lokalen Ultraschallsensorwerts als Eingabeparameter. In den Jahren 2013 und 2014 wurden Feldversuche in Winterweizen durchgeführt, um die Beziehung zwischen den Sensorwerten (Ultraschall- und Kamerasensor) und den Pflanzenparametern Pflanzenoberfläche (Leaf Area Index, LAI) sowie Biomasse zu analysieren. Diese sind für einen örtlich angepassten variablen Fungizideinsatz zur Bemessung der Spritzmenge wichtig. Die Messungen wurden mehrmals während der Vegetationsperiode an visuell ausgewählten Stichprobenpunkten entsprechend der unterschiedlichen Bestandsdichte durchgeführt. Nach Änderungen an der Sensortechnik konnten für 2014 signifikante lineare Regressionsmodelle zur Beschreibung der Beziehung zwischen den Sensorwerten und den zwei Pflanzenparametern LAI sowie Biomasse gefunden werden.
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    Complete Genome Sequence of a New Firmicutes Species Isolated from Anaerobic Biomass Hydrolysis
    (Washington, DC : American Soc. for Microbiology, 2017) Abendroth, Christian; Hahnke, Sarah; Codoñer, Francisco M.; Klocke, Michael; Luschnig, Olaf; Porcar, Manuel
    A new Firmicutes isolate, strain HV4-6-A5C, was obtained from the hydrolysis stage of a mesophilic and anaerobic two-stage lab-scale leach-bed system for biomethanation of fresh grass. It is assumed that the bacterial isolate contributes to plant biomass degradation. Here, we report a draft annotated genome sequence of this organism. © 2017 Abendroth et al.
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    Role of biogas and biochar palm oil residues for reduction of greenhouse gas emissions in the biodiesel production
    (Amsterdam : Elsevier, 2015) Harsono, Soni Sisbudi; Grundmann, Philipp; Siahaanc, Donald
    Greenhouse gas (GHG) emissions which related to palm oil production are tend to increase due to the increasing of palm oil demand and the expansion process of oil palm production worldwide. The specific objective of the study was to assess the contribution of innovative biomass processes as effort to improve the energy balance and reduce the greenhouse gas emissions (GHG) associated with biodiesel made from palm oil. The GHG was calculated that GHG emission savings up to 63.14% in total. GHG emissions from biochar using empty fruit bunches (EFB) resulted to 2.95% from total GHG emissions, and biogas from palm oil mill effluent (POME) produced 74.22% of the total GHG emissions from palm oil based biodiesel production. Innovative technologies and processes for the treatment of by-products can contribute significantly for meeting the emission targets. Build upon the research, resulted to the recommendation to use biochar and capturing methane from POME. The research result was also concerned that emission savings are annulled in the case of land use change (LUC) and oil palm production on peatland. Based on this research resulted to recommended that the utilization of waste from oil palm cultivation on peatland which was disuse and the capturing of methane from POME
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    CUDe — Carbon utilization degree as an indicator for sustainable biomass use
    (Basel : MDPI, 2016) Anja Hansen, Anja Hansen; Budde, Jörn; Karatay, Yusuf Nadi; Prochnow, Annette
    Carbon (C) is a central element in organic compounds and is an indispensable resource for life. It is also an essential production factor in bio-based economies, where biomass serves many purposes, including energy generation and material production. Biomass conversion is a common case of transformation between different carbon-containing compounds. At each transformation step, C might be lost. To optimize the C use, the C flows from raw materials to end products must be understood. The estimation of how much of the initial C in the feedstock remains in consumable products and delivers services provides an indication of the C use efficiency. We define this concept as Carbon Utilization Degree (CUDe) and apply it to two biomass uses: biogas production and hemp insulation. CUDe increases when conversion processes are optimized, i.e., residues are harnessed and/or losses are minimized. We propose CUDe as a complementary approach for policy design to assess C as an asset for bio-based production. This may lead to a paradigm shift to see C as a resource that requires sustainable exploitation. It could complement the existing methods that focus solely on the climate impact of carbon.